Rapid separation of particulate components and soluble cytoplasm of isolated rat-liver cells.
Rapid separation of particulate components and soluble cytoplasm of isolated rat-liver cells.
复制标题
快速分离分离的大鼠肝细胞的颗粒成分和可溶性细胞质。
DOI：
10.1016/0005-2728(74)90022-x
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发表时间：
1974
期刊：
影响因子：
--
通讯作者：
J. Tager
中科院分区：
文献类型：
--
作者：
P. Zuurendonk;J. Tager
描述了一种从分离的大鼠肝细胞的可溶性细胞质中快速分离线粒体（加上其他颗粒成分）的方法。将细胞与低浓度的洋地黄皂苷短暂孵育。快速离心后，沉淀含有超过 90% 的总腺苷酸激酶和谷氨酸脱氢酶活性，上清液含有至少 80% 的乳酸脱氢酶活性。肝细胞中约60%的总腺嘌呤核苷酸存在于可溶性细胞质中。代谢丙氨酸的肝细胞暴露于洋地黄皂苷后80 s，颗粒部分中的ATP/ADP比为2.0-2.4，可溶性细胞质中的ATP/ADP比为6-19。在存在苍术苷的情况下，这些值分别为 3.5-4.4 和 1.3-2.2。
A method is described for the rapid separation of mitochondria (plus other particulate components) from the soluble cytoplasm of isolated rat-liver cells. The cells were incubated briefly with a low concentration of digitonin. After rapid centrifugation, the pellet contained more than 90% of the total adenylate kinase and glutamate dehydrogenase activities and the supernatant at least 80% of the lactate dehydrogenase activity. About 60% of total adenine nucleotides in hepatocytes were found in the soluble cytoplasm. The ATP/ADP ratio in the particulate fraction 80 s after exposure to digitonin of hepatocytes metabolizing alanine was 2.0–2.4, and that in the soluble cytoplasm 6–19. In the presence of atractyloside, these values were 3.5–4.4 and 1.3–2.2, respectively.