Comparison of three diagnostic methods (microscopy, RDT, and PCR) for the detection of malaria parasites in representative samples from Equatorial Guinea.
Comparison of three diagnostic methods (microscopy, RDT, and PCR) for the detection of malaria parasites in representative samples from Equatorial Guinea.
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在赤道几内亚的代表性样本中检测三种诊断方法（显微镜，RDT和PCR）的三种诊断方法（显微镜，RDT和PCR）的比较。
DOI：
10.1186/s12936-018-2481-4
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发表时间：
2018-09-17
期刊：
影响因子：
3
通讯作者：
Benito A
中科院分区：
文献类型：
--
作者：
Berzosa P;de Lucio A;Romay-Barja M;Herrador Z;González V;García L;Fernández-Martínez A;Santana-Morales M;Ncogo P;Valladares B;Riloha M;Benito A
赤道几内亚的疟疾仍然是一个主要的公共卫生问题。该国是全息流行区，具有全年传播模式。 2016年，疟疾患病率为12.09%，疟疾导致5岁以下儿童死亡的15%。在大陆地区，2011年，95.2%的疟疾感染是恶性疟原虫，9.5%是间日疟原虫，混合感染8例。疟疾控制的主要策略是快速、准确的诊断和有效的治疗。疟疾的早期和准确诊断对于有效的疾病管理和疟疾监测至关重要。疟疾诊断的质量在所有情况下都很重要，因为误诊可能导致显着的发病率和死亡率。显微镜和 RDT 是现场诊断疟疾的主要选择。然而，假阴性结果可能会延误治疗并增加社区中能够感染蚊子的人数。本研究分析了显微镜和 RDT（赤道几内亚用于诊断疟疾的两种主要技术）与半巢式多重 PCR (SnM-PCR) 的性能比较。通过显微镜、RDT 和 SnM-PCR 检测了总共 1724 个样本。显微镜检出的阴性样本中，有335份（19.4%）为假阴性。另一方面，RDT检测到的阴性样本中有128个（13.3%）是基于PCR的假阴性。这一发现很重要，特别是因为这是一组没有接受抗疟治疗的患者。由于显微镜检查假阴性率较高，有必要加强对流行地区“黄金标准”显微镜检查的培训。从长远来看，参考中心网络可能会支持疟疾控制计划正在进行的诊断和控制工作，因为国家热带医学中心目前支持赤道几内亚国家防治疟疾计划进行疾病控制所需的所有分子研究。考虑到 RDT 获得的结果，必须在 EG 中对 hrp2 基因的删除进行详尽的研究，以帮助为该区域选择正确的 RDT。
Malaria in Equatorial Guinea remains a major public health problem. The country is a holo-endemic area with a year-round transmission pattern. In 2016, the prevalence of malaria was 12.09% and malaria caused 15% of deaths among children under 5 years. In the Continental Region, 95.2% of malaria infections were Plasmodium falciparum, 9.5% Plasmodium vivax, and eight cases mixed infection in 2011. The main strategy for malaria control is quick and accurate diagnosis followed by effective treatment. Early and accurate diagnosis of malaria is essential for both effective disease management and malaria surveillance. The quality of malaria diagnosis is important in all settings, as misdiagnosis can result in significant morbidity and mortality. Microscopy and RDTs are the primary choices for diagnosing malaria in the field. However, false-negative results may delay treatment and increase the number of persons capable of infecting mosquitoes in the community. The present study analysed the performance of microscopy and RDTs, the two main techniques used in Equatorial Guinea for the diagnosis of malaria, compared to semi-nested multiplex PCR (SnM-PCR). A total of 1724 samples tested by microscopy, RDT, and SnM-PCR were analysed. Among the negative samples detected by microscopy, 335 (19.4%) were false negatives. On the other hand, the negative samples detected by RDT, 128 (13.3%) were false negatives based on PCR. This finding is important, especially since it is a group of patients who did not receive antimalarial treatment. Owing to the high number of false negatives in microscopy, it is necessary to reinforce training in microscopy, the “Gold Standard” in endemic areas. A network of reference centres could potentially support ongoing diagnostic and control efforts made by malaria control programmes in the long term, as the National Centre of Tropical Medicine currently supports the National Programme against Malaria of Equatorial Guinea to perform all of the molecular studies necessary for disease control. Taking into account the results obtained with the RDTs, an exhaustive study of the deletion of the hrp2 gene must be done in EG to help choose the correct RDT for this area.
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3
作者：
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通讯作者：
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影响因子：
3
作者：
Abeku TA;Kristan M;Jones C;Beard J;Mueller DH;Okia M;Rapuoda B;Greenwood B;Cox J
通讯作者：
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