Photonics probing of pup brain tissue and molecular-specific nuclear nanostructure alterations due to fetal alcoholism via light scattering/localization approaches.
Photonics probing of pup brain tissue and molecular-specific nuclear nanostructure alterations due to fetal alcoholism via light scattering/localization approaches.
复制标题
DOI：
10.1117/1.jbo.27.7.076002
复制
发表时间：
2022-07
影响因子：
3.5
通讯作者：
Pradhan, Prabhakar
中科院分区：
文献类型：
--
作者：
Adhikari, Prakash;Shukla, Pradeep;Alharthi, Fatemah;Bhandari, Shiva;Meena, Avtar;Rao, Radhakrishna;Pradhan, Prabhakar
关键词：
光是一种很好的探针，用于研究由于压力，酒精或任何其他异常而导致的脑细胞/组织的纳米级结构或分子特异性结构特性。怀孕期间的慢性酒精中毒，即，胎儿酒精中毒具有致畸性，导致胎儿酒精综合征和其他神经系统疾病。了解纳米到亚微米尺度的空间结构特性的幼犬脑细胞/组织使用光/光子探针可以提供大量的信息，了解胎儿酒精中毒的影响。使用光散射和光定位技术来探测暴露于胎儿酒精中毒的小鼠幼仔的脑细胞/组织中的纳米至亚微米尺度质量密度或折射率波动的改变。我们使用介观物理为基础的双光谱成像技术，部分波谱（PWS）和分子特异性的逆参与比（IPR），使用共聚焦成像，以量化脑组织和脑细胞中的染色质/组蛋白的结构改变，分别在出生后60天的小鼠幼鼠大脑，暴露于胎儿酒精中毒。对组织的更精细聚焦PWS分析显示幼鼠脑组织中结构紊乱强度的程度增加。此外，分子特异性光定位IPR技术的结果显示，DNA中的空间分子质量密度结构无序程度增加，组蛋白中的程度降低。特别是，我们从分子到组织水平的空间幼犬脑结构的特点，并解决合理的原因，如质量密度波动胎儿酒精中毒。
Light is a good probe for studying the nanoscale-level structural or molecular-specific structural properties of brain cells/tissue due to stress, alcohol, or any other abnormalities. Chronic alcoholism during pregnancy, i.e., fetal alcoholism, being teratogenic, results in fetal alcohol syndrome, and other neurological disorders. Understanding the nano-to-submicron scale spatial structural properties of pup brain cells/tissues using light/photonic probes could provide a plethora of information in understanding the effects of fetal alcoholism. Using both light scattering and light localization techniques to probe alterations in nano- to-submicron scale mass density or refractive index fluctuations in brain cells/tissues of mice pups, exposed to fetal alcoholism. We use the mesoscopic physics-based dual spectroscopic imaging techniques, partial wave spectroscopy (PWS) and molecular-specific inverse participation ratio (IPR) using confocal imaging, to quantify structural alterations in brain tissues and chromatin/histone in brain cells, respectively, in 60 days postnatal mice pup brain, exposed to fetal alcoholism. The finer focusing PWS analysis on tissues shows an increase in the degree of structural disorder strength in the pup brain tissues. Furthermore, results of the molecular-specific light localization IPR technique show an increase in the degree of spatial molecular mass density structural disorder in DNA and a decrease in the degree in histone. In particular, we characterize the spatial pup brain structures from the molecular to tissue levels and address the plausible reasons for such as mass density fluctuations in fetal alcoholism.