Expression of the c-fos-monomeric red fluorescent protein l fusion gene in the spinal cord and the hypothalamic paraventricular nucleus in transgenic rats after formalin injection
Expression of the c-fos-monomeric red fluorescent protein l fusion gene in the spinal cord and the hypothalamic paraventricular nucleus in transgenic rats after formalin injection
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福尔马林注射后转基因大鼠脊髓和下丘脑室旁核c-fos单体红色荧光蛋白l融合基因的表达
DOI：
10.1016/j.brainres.2012.08.033
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发表时间：
2012
期刊：
影响因子：
2.9
通讯作者：
Y
中科院分区：
文献类型：
--
作者：
Ishikura;T. Suzuki;H. Yoshimura;M. Ohkubo;J. Katoh;A. Ohbuchi;T. Ohno;M.Fujihara;H. Kawasaki;M. Ohnishi,H. Nakamura;T. & Ueta;Y
我们培育了经过充分刺激后在中枢神经系统中表达 c-fos 和单体红色荧光蛋白 1 (mRFP1) 融合基因的转基因大鼠。在本研究中，在双后爪皮下（s.c.）注射0.9％盐水和5％福尔马林后24小时内，将脊髓和室旁核（PVN）中mRFP1荧光诱导模式的时程与Fos样免疫反应性（LI）的时程进行比较。对照大鼠不进行治疗。在对照组和注射生理盐水/福尔马林的大鼠中，0分钟时在脊髓和PVN中观察到分散的mRFP1荧光，但同一区域中几乎没有Fos-LI。福尔马林后3小时，脊髓和PVN中的mRFP1荧光增加。另一方面，福尔马林后脊髓和PVN中Fos-LI的变化相对于mRFP1荧光的变化较短。这些结果表明，c-fos-mRFP1融合基因表达在正常条件下略有上调，并且伤害性刺激诱导的融合基因的诱导可能比脊髓和PVN中的内源性c-fos基因表达维持更长时间。接下来，皮下注射后脊髓和 PVN 中的伤害行为、mRFP1 荧光和 Fos-LI。比较注射福尔马林、4α-佛波醇 12,13-二癸酸酯 (4α-PDD) 和生理盐水。尽管注射 4α-PDD 的大鼠很少表现出像皮下注射那样的伤害行为。与皮下注射不同，盐水注射、4α-PDD注射在脊髓和PVN中显着引起mRFP1荧光和Fos-LI。生理盐水注射。
We generated transgenic rats expressing the c-fos and monomeric red fluorescent protein 1 (mRFP1) fusion gene in the central nervous system after adequate stimulation. In the present study, the time-course of the induction patterns of mRFP1 fluorescence in the spinal cord and the paraventricular nucleus (PVN) was compared with that of Fos-like immunoreactivity (LI) within 24h after subcutaneous (s.c.) injection of 0.9% saline and 5% formalin in both hind paws. Control rats were not treated. In the control and saline/formalin injected rats, scattered mRFP1 fluorescence in the spinal cord and the PVN was observed at 0min, though there was little Fos-LI in the same region. The mRFP1 fluorescence in the spinal cord and the PVN was increased at 3h after formalin. On the other hand, the changes of Fos-LI in the spinal cord and the PVN were relatively shorter than those of the mRFP1 fluorescence after formalin. These results suggest that the c-fos-mRFP1 fusion gene expression is slightly upregulated in normal conditions and nociceptive stimulation-induced induction of the fusion gene may be maintained longer than the endogenous c-fos gene expression in the spinal cord and the PVN. Next, nocifensive behavior and mRFP1 fluorescence and Fos-LI in the spinal cord and the PVN after s.c. injection of formalin, 4α-phorbol 12,13-didecanoate (4α-PDD) and saline were compared. Although the 4α-PDD injected rats seldom displayed nocifensive behaviors like s.c. saline injection, 4α-PDD injection caused mRFP1 fluorescence and Fos-LI significantly in the spinal cord and the PVN unlike s.c. saline injection.