A novel Arabidopsis gene causes Bax-like lethality in Saccharomyces cerevisiae
A novel Arabidopsis gene causes Bax-like lethality in Saccharomyces cerevisiae
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DOI：
10.1074/jbc.m509632200
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发表时间：
2005-11-25
影响因子：
4.8
通讯作者：
Uchimiya, H
中科院分区：
文献类型：
--
作者：
Kawai-Yamada, M;Saito, Y;Uchimiya, H
哺乳动物促凋亡蛋白Bax的过表达诱导植物和酵母细胞中的细胞死亡。Bax inhibitor-1（BI-1）基因可使酵母和植物免于由Bax介导的致死。利用GAL 1启动子控制的拟南芥BI-1（AtBI-1）基因作为酵母细胞死亡抑制因子，从拟南芥cDNA文库中分离到Cdf 1（（c）在bar下细胞生长（d）在bar下作用（f）在bar下作用-（1）在bar下）。Cdf 1的过表达导致酵母细胞死亡，而这种效果被AtBI-1的共表达抑制。Cdf 1蛋白融合绿色荧光蛋白定位在线粒体中，并导致在酵母线粒体膜电位的损失。抗Cdf 1的突变体BRM 1表现出对Cdf 1介导的致死性的耐受性，而Δ atp 4菌株对Cdf 1敏感。我们的研究结果表明，Cdf 1和Bax通过部分重叠的途径引起酵母介导的酵母致死。
Overexpression of the mammalian proapoptotic protein Bax induces cell death in plant and yeast cells. The Bax inihibitor-1 (BI-1) gene rescues yeast and plant from Bax-mediated lethality. Using the Arabidopsis BI-1 (AtBI-1) gene controlled by the GAL1 promoter as a cell death suppressor in yeast, Cdf1 ((c) under bar ell growth (d) under bar efect (f) under bar actor-(1) under bar) was isolated from Arabidopsis cDNA library. Overexpression of Cdf1 caused cell death in yeast, whereas such an effect was suppressed by co-expression of AtBI-1. The Cdf1 protein fused with a green fluorescent protein was localized in the mitochondria and resulted in the loss of mitochondrial membrane potential in yeast. The Bax-resistant mutant BRM1 demonstrated tolerance against Cdf1-mediated lethality, whereas the Delta atp4 strain was sensitive to Cdf1. Our results suggest that Cdf1 and Bax cause mitochondria-mediated yeast lethality through partially overlapped pathways.