Classification Algorithm for Subspecies Identification within the Mycobacterium abscessus Species, Based on Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
Classification Algorithm for Subspecies Identification within the Mycobacterium abscessus Species, Based on Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
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DOI：
10.1128/jcm.00788-14
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发表时间：
2014-09-01
影响因子：
9.4
通讯作者：
Hery-Arnaud, Genevieve
中科院分区：
文献类型：
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作者：
Fangous, Marie-Sarah;Mougari, Faiza;Hery-Arnaud, Genevieve
结核分枝杆菌作为一个物种，越来越多地涉及呼吸道感染，特别是在囊性纤维化患者中。该物种包括3个亚种，可能难以识别。由于它们在抗生素敏感性和临床相关性方面存在差异，因此开发一种在亚种水平上区分结核分枝杆菌的常规诊断工具是一个真实的挑战。采用基质辅助激光解吸电离飞行时间质谱法（MALDI-TOF MS）对43株先前通过多位点序列分型鉴定的分枝杆菌分离株进行了分析。一个亚种识别算法，根据5个鉴别峰，制定和验证的盲法鉴定另外49株，其中94%（n = 46）被正确识别。两米红腹叶蝉亚种结果表明，该菌株被误认为M.红腹叶蝉亚种其他1株菌株鉴定失败。实验室间和实验室内的重现性测试是结论性的。本研究首次提出了一种用于MALDI-TOF MS鉴定3 M的分类算法。abscessus subspecies. MALDI-TOF MS能有效区分M.微生物物种，可以很容易地融入微生物学实验室的工作流程。
Mycobacterium abscessus, as a species, has been increasingly implicated in respiratory infections, notably in cystic fibrosis patients. The species comprises 3 subspecies, which can be difficult to identify. Since they differ in antibiotic susceptibility and clinical relevance, developing a routine diagnostic tool discriminating Mycobacterium abscessus at the subspecies level is a real challenge. Forty-three Mycobacterium abscessus species isolates, previously identified by multilocus sequence typing, were analyzed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). A subspecies identification algorithm, based on five discriminating peaks, was drawn up and validated by blind identification of a further 49 strains, 94% of which (n = 46) were correctly identified. Two M. abscessus subsp. massiliense strains were misidentified as M. abscessus subsp. abscessus, and for 1 other strain identification failed. Inter-and intralaboratory reproducibility tests were conclusive. This study presents, for the first time, a classification algorithm for MALDI-TOF MS identification of the 3 M. abscessus subspecies. MALDI-TOF MS proved effective in discriminating within the M. abscessus species and might be easily integrated into the work-flow of microbiology labs.