Bioluminescent system for dynamic imaging of cell and animal behavior
Bioluminescent system for dynamic imaging of cell and animal behavior
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DOI：
10.1016/j.bbrc.2012.01.141
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发表时间：
2012-03-09
影响因子：
3.1
通讯作者：
Okano, Hideyuki
中科院分区：
文献类型：
--
作者：
Hara-Miyauchi, Chikako;Tsuji, Osahiko;Okano, Hideyuki
目前生物发光成像的实用性受到光子产量低的限制，从而限制了时间敏感性。在这里，我们描述了一种使用化学发光/荧光蛋白 ffLuc-cp156 的成像方法，该蛋白由水母 GFP 的黄色变体和萤火虫荧光素酶组成。我们报告说，与当前的生物发光系统相比，光子产量提高了三个数量级以上。我们以高分辨率对细胞运动进行成像，包括神经元生长锥和小胶质细胞突起。转基因 ffLuc-cp156 小鼠能够对自由活动的动物进行视频速率生物发光成像，这可能为临床前研究的行为动物中的药物分布提供可靠的测定。 (C) 2012 Elsevier Inc. 保留所有权利。
The current utility of bioluminescence imaging is constrained by a low photon yield that limits temporal sensitivity. Here, we describe an imaging method that uses a chemiluminescent/fluorescent protein, ffLuc-cp156, which consists of a yellow variant of Aequorea GFP and firefly luciferase. We report an improvement in photon yield by over three orders of magnitude over current bioluminescent systems. We imaged cellular movement at high resolution including neuronal growth cones and microglial cell protrusions. Transgenic ffLuc-cp156 mice enabled video-rate bioluminescence imaging of freely moving animals, which may provide a reliable assay for drug distribution in behaving animals for pre-clinical studies. (C) 2012 Elsevier Inc. All rights reserved.