ANALYSIS OF CELLS OBTAINED BY BRONCHIAL LAVAGE OF INFANTS WITH RESPIRATORY SYNCYTIAL VIRUS-INFECTION
ANALYSIS OF CELLS OBTAINED BY BRONCHIAL LAVAGE OF INFANTS WITH RESPIRATORY SYNCYTIAL VIRUS-INFECTION
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DOI：
10.1136/adc.71.5.428
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发表时间：
1994-11-01
影响因子：
5.2
通讯作者：
MILNER, AD
中科院分区：
文献类型：
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作者：
EVERARD, ML;SWARBRICK, A;MILNER, AD
为研究呼吸道合胞病毒毛细支气管炎患儿的呼吸道细胞渗出情况，采用鼻咽吸入器对呼吸道合胞病毒毛细支气管炎患儿进行下呼吸道和非插管患儿上呼吸道的呼吸道分泌物标本的支气管灌洗，制备细胞体标本，进行细胞分类计数，并用抗CD3、抗CD4、抗CD8、抗CD19、抗TCR-γβ的单抗对淋巴细胞亚群进行免疫细胞化学分析。获得大量炎性细胞，其中中性粒细胞在上呼吸道占93%，在下呼吸道占76%。CD8阳性细胞数较少，且始终少于CD4阳性细胞，上、下呼吸道的中位数分别为22.5：1和15：1。CD19阳性细胞很少，也未检测到γ-β阳性淋巴细胞。这些结果表明，中性粒细胞可能在这些婴儿的症状中起主要作用。他们不支持过多的淋巴细胞介导的细胞毒活性是呼吸道合胞病毒毛细支气管炎病理的主要原因的观点。
To study the cellular infiltrate that occurs within the airways of infants with respiratory syncytial virus bronchiolitis, samples of airways secretions were obtained by bronchial lavage from the lower respiratory tract of infants ventilated for this condition and from the upper airway of non-intubated infants with this disorder using nasopharyngeal aspirates.Cytospin samples were prepared so that differential cell counts could be performed on the cells obtained and alkaline phosphatase-antialkaline phosphatase immunocytochemical analysis of lymphocyte subsets was carried out using a panel of monoclonal antibodies, which included anti-CD3, anti-CD4, anti-CD8, anti-CD19, and anti-TcR gamma delta.Results from the lower and upper airways were similar. Large numbers of inflammatory cells were obtained, of which neutrophils accounted for a median of 93% in the upper airway and 76% in the lower airway. The numbers of CD8 positive cells detected were small and consistently less than CD4 positive cells, median CD4:CD8 ratios being 22.5:1 and 15:1 for the lower and upper airways. CD19 positive cells were rarely observed and no gamma delta positive lymphocytes were detected.These results indicate that neutrophils probably play a major part in causing symptoms in these infants. They do not support the concept that excessive lymphocyte mediated cytotoxic activity is principally responsible for the pathology in respiratory syncytial virus bronchiolitis.