Transformation Frequency of a mariner-Based Transposon in Rickettsia rickettsii
Transformation Frequency of a mariner-Based Transposon in Rickettsia rickettsii
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DOI：
10.1128/jb.05279-11
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发表时间：
2011-09-01
影响因子：
3.2
通讯作者：
Hackstadt, Ted
中科院分区：
文献类型：
--
作者：
Clark, Tina R.;Lackey, Amanda M.;Hackstadt, Ted
利用噬斑试验系统对立氏立克次体中基于mariner的转座子系统的转化频率进行了测定，以用于突变体的计数和分离。序列分析表明，两种R. rickettsii和R. prowazekii指出插入是随机的。转座子突变为立克次体的研究提供了一个有用的工具。
Transformation frequencies of a mariner-based transposon system in Rickettsia rickettsii were determined using a plaque assay system for enumeration and isolation of mutants. Sequence analysis of insertion sites in both R. rickettsii and R. prowazekii indicated that insertions were random. Transposon mutagenesis provides a useful tool for rickettsial research.