EVIDENCE THAT TETRACYCLINE ANALOGS WHOSE PRIMARY TARGET IS NOT THE BACTERIAL RIBOSOME CAUSE LYSIS OF ESCHERICHIA-COLI
EVIDENCE THAT TETRACYCLINE ANALOGS WHOSE PRIMARY TARGET IS NOT THE BACTERIAL RIBOSOME CAUSE LYSIS OF ESCHERICHIA-COLI
复制标题
DOI：
10.1128/aac.36.5.913
复制
发表时间：
1992-05-01
影响因子：
4.9
通讯作者：
CHOPRA, I
中科院分区：
文献类型：
--
作者：
OLIVA, B;GORDON, G;CHOPRA, I
研究了不直接抑制细菌蛋白合成的非典型四环素的作用模式。所测试的类似物chelocardin、无水四环素、6-噻唑四环素、无水四环素和4-外皮无水四环素具有杀菌作用，并引起大肠杆菌裂解，同时细胞质酶-半乳糖苷酶释放到上清液中。电子显微镜检查表明，暴露于这些类似物的细胞发生了明显的形态学改变，包括在培养基中形成许多鬼影和细胞碎片的出现。尽管非典型四环素促进了完整生物体的裂解，但它们并没有引起大肠杆菌球质体的裂解，这表明类似物不会直接破坏细胞质膜。这些药物可能通过干扰膜的电化学梯度来促进细胞的裂解和死亡，从而导致自溶酶活性的刺激和细胞的裂解。该结果支持最近公布的数据，该数据表明四环素根据其作用方式可分为两类。
The modes of action of atypical tetracyclines that do not directly inhibit bacterial protein synthesis were investigated. The analogs tested, chelocardin, anhydrotetracycline, 6-thiatetracycline, anhydrochlortetracycline, and 4-epi-anhydrochlortetracycline, were bactericidal and caused the lysis of Escherichia coli accompanied by the release of the cytoplasmic enzyme beta-galactosidase into the supernatant. Examination by electron microscopy demonstrated that cells exposed to these analogs underwent marked morphological alterations that included the formation of numerous ghosts and the appearance of cellular debris in the culture medium. Although atypical tetracyclines promoted lysis in intact organisms, they did not cause lysis of E. coli spheroplasts, indicating that the analogs do not directly destroy the cytoplasmic membrane. These agents may promote cell lysis and death by interfering with the membrane's electrochemical gradient, which in turn leads to stimulation of autolytic enzyme activity and cellular lysis. The results support recently published data which indicate that tetracyclines are divisible into two classes on the basis of their modes of action.