PLASMODIUM-FALCIPARUM GAMETOCYTOGENESIS INVITRO
PLASMODIUM-FALCIPARUM GAMETOCYTOGENESIS INVITRO
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DOI：
10.1038/264271a0
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发表时间：
1976-01-01
期刊：
影响因子：
64.8
通讯作者：
SMALLEY, ME
中科院分区：
文献类型：
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作者：
SMALLEY, ME
疟疾寄生虫的有性繁殖机制正受到越来越多的关注。电子显微镜1和动力学研究2正在阐明配子发生。另一方面，尽管有早期的工作3-5，配子细胞发生仍然知之甚少。恶性疟原虫配子细胞的发育特别令人感兴趣，因为它们在人类的深层组织空间中发育，尤其是脾脏和骨髓。未成熟阶段很少出现在外周血中，因此迄今为止尚未进行详细的实验研究。此外，长期以来人们一直怀疑它们的发育被延长5，需要8-12天，尽管也有相反的建议6,7。 P. 未成熟的配子体最近在被认为仅由无性寄生虫组成的文化中发现了恶性疟原虫8。我使用了类似的微培养技术，该技术允许在体外培养形态成熟的P。恶性疟原虫配子细胞，并在此提出对恶性疟原虫配子细胞发生过程的一些初步观察。恶性疟原虫感染。
THE mechanism of sexual reproduction among malaria parasites is coming under increasing scrutiny. Gametogenesis is being unravelled by electron microscopy1and by kinetic studies2. Gametocytogenesis on the other hand remains poorly understood in spite of earlier work3–5. The development ofPlasmodium falciparumgametocytes is particularly intriguing because in man they develop in the deep tissue spaces, especially the spleen and bone marrow. The immature stages only rarely appear in the peripheral blood and as a result have escaped detailed experimental investigation until now. Furthermore, it has long been suspected that their development is prolonged5, taking 8–12 d, although there are suggestions to the contrary6,7. Immature gametocytes ofP. falciparumhave recently been reported in cultures thought to be composed only of asexual parasites8. I have used a similar microculture technique which has permitted the developmentin vitroof morphologically matureP. falciparumgametocytes and present here some preliminary observations on the process of gametocytogenesis inP. falciparuminfections.