Photosensitive Hydrogel Creates Favorable Biologic Niches to Promote Spinal Cord Injury Repair
Photosensitive Hydrogel Creates Favorable Biologic Niches to Promote Spinal Cord Injury Repair
复制标题
光敏水凝胶创造有利的生物位促进脊髓损伤修复
DOI：
10.1002/adhm.201900013
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发表时间：
2019-07-01
影响因子：
10
通讯作者：
Zhu, Linyong
中科院分区：
文献类型：
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作者：
Cai, Zhengwei;Gan, Yibo;Zhu, Linyong
光化学被认为是水凝胶模拟天然细胞外基质的复杂和动态性质的一种有前途的策略。但由于光的衰减，它在三维组织工程和再生医学中的应用很少。在这项研究中，叠氮苯光化学和光纤技术首次用于定位在三维水凝胶支架中的神经引导导管的内表面上的粘附蛋白。体外神经干细胞共培养实验表明，胶原蛋白光固定能显著提高神经干细胞在管道中的粘附和存活，并与生长因子的持续释放具有协同作用。植入横断脊髓后，发现优化的水凝胶支架可改善脊髓损伤（SCI）后12周大鼠的运动恢复。组织学分析表明，所设计的水凝胶支架为神经元再生提供了有利的生物小生境，从而产生定向神经元组织并促进SCI的修复。这项研究证明了一个有前途的水凝胶支架SCI修复，并提供了第一次了解的光固定的粘附蛋白在3D水凝胶导管有关其功能的脊髓组织修复。
Photochemistry is considered to be a promising strategy for hydrogels to mimic the complex and dynamic properties of natural extracellular matrix. However, it is seldom applied in 3D tissue engineering and regenerative medicine due to the attenuation of light. In this study, phenyl azide photchemistry and optical fiber technology are first used to localize adhesive protein on the inner surface of the nerve guidance conduit in a 3D hydrogel scaffold. In vitro coculture assay of neural stem cells (NSCs) shows that photoimmobilization of collagen significantly improves the adhesion and survival of NSCs in the conduit, and exhibits synergistic effect with the sustainable release of growth factor. After implantation in transected spinal cord, the optimized hydrogel scaffold is found to improve the locomotion recovery of rats 12 weeks after spinal cord injury (SCI). Histological analysis suggests that the designed hydrogel scaffold provides a favorable biological niche for neuronal regeneration, thus producing directional neuron tissue and promoting the repair of SCI. This study demonstrates a promising hydrogel scaffold for SCI repair and provides the first understanding of the photoimmobilization of adhesive protein in a 3D hydrogel conduit concerning its functions on spinal cord tissue restoration.