CELL-MEDIATED IMMUNE-RESPONSE TO HUMAN-IMMUNODEFICIENCY-VIRUS (HIV) TYPE-1 IN SERONEGATIVE HOMOSEXUAL MEN WITH RECENT SEXUAL EXPOSURE TO HIV-1
CELL-MEDIATED IMMUNE-RESPONSE TO HUMAN-IMMUNODEFICIENCY-VIRUS (HIV) TYPE-1 IN SERONEGATIVE HOMOSEXUAL MEN WITH RECENT SEXUAL EXPOSURE TO HIV-1
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DOI：
10.1093/infdis/165.6.1012
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发表时间：
1992-06-01
影响因子：
6.4
通讯作者：
SHEARER, GM
中科院分区：
文献类型：
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作者：
CLERICI, M;GIORGI, JV;SHEARER, GM
虽然人类免疫缺陷病毒（HIV）1型感染通过性交有效传播，但一些性行为使其处于极高感染风险的个体仍然保持HIV-1血清阴性。进行了一项调查，以确定这些人是否有对HIV-1敏感的循环T辅助细胞。研究了5名最近发生过HIV-1性接触的高危男性。来自所有5个的外周血单个核细胞在培养物中响应于合成的两亲性HIV-1包膜肽产生白细胞介素（IL）-2。5名高危男性中的1名随后发生血清转化，而4名仍为血清阴性。所有患者最初的培养结果均为阴性，而那些血清反应阴性的患者在首次研究后10个月通过聚合酶链反应（PCR）检测也为病毒阴性。这些结果表明，在最近暴露于HIV-1的个体中不存在体液免疫应答的情况下，可以检测到对HIV-1的细胞介导的免疫应答。此外，T细胞对合成肽的反应产生IL-2可能是比抗体、淋巴细胞增殖或PCR测试更敏感的暴露于HIV-1的测试。
Although human immunodeficiency virus (HIV) type 1 infection is efficiently transmitted by sexual intercourse, some individuals whose sexual behavior places them at extremely high risk for infection have nevertheless remained HIV-1-seronegative. An investigation was undertaken to determine whether such individuals have circulating T helper cells that are sensitized to HIV-1. Five very high risk men who had recent sexual exposure to HIV-1 were studied. Peripheral blood mononuclear cells from all 5 produced interleukin (IL)-2 in culture in response to synthetic amphipathic HIV-1 envelope peptides. One of the 5 high-risk men has subsequently seroconverted, while 4 have remained seronegative. All were initially culture-negative, and those who have remained seronegative were also virus-negative by polymerase chain reaction (PCR) testing 10 months after they were first studied. These results demonstrate that a cell-mediated immune response to HIV-1 can be detected in the absence of a humoral immune response in individuals recently exposed to HIV-1. Furthermore, IL-2 production by T cells in response to synthetic peptides may be a more sensitive test for exposure to HIV-1 than antibody, lymphoproliferation, or PCR tests.