The Caco-2 cell culture system can be used as a model to study food iron availability
The Caco-2 cell culture system can be used as a model to study food iron availability
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DOI：
10.1093/jn/126.1.251
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发表时间：
1996-01-01
影响因子：
4.2
通讯作者：
Cook, JD
中科院分区：
文献类型：
--
作者：
Garcia, MN;Flowers, C;Cook, JD
为了评估Caco-2细胞培养系统作为研究膳食铁利用率的模型的有效性，进行了初步实验，以确定铁摄取和运输的最佳条件。在pH为5.5、抗坏血酸与铁的摩尔比为2：1、孵育时间为1h的条件下，放射性硫酸亚铁的铁摄取率最高。在此实验条件下，我们研究了添加不同肉源匀浆、大豆分离蛋白、蛋清和牛血清白蛋白上清液对铁吸收的影响。肉类的铁摄取率为6.3+/-1.7%，显著高于大豆蛋白的1.2+/-0.3%、蛋清的1.3+/-0.3%和牛血清白蛋白的0.8+/-0.1%(P<；0.001)。当蛋白质浓度平衡时，消化的肉类样品的铁摄取量也显著高于未消化的肉类样品。从肉类和大豆蛋白提取物制备等电聚焦后得到的级分中对铁的吸收和蛋白质浓度的测量表明，肉类中的蛋白质浓度和铁吸收的两个峰明显不存在于大豆蛋白中，这两个峰中含有导致细胞铁吸收较高的因素(S)。鉴于这些观察到的与人体铁吸收研究的相似之处，我们得出结论，Caco-2细胞培养系统是研究食物铁利用率的有用的体外模型。
To assess the usefulness of the Caco-2 cell culture system as a model to study the availability of dietary iron, preliminary experiments were performed to determine the optimal conditions for iron uptake and transport. Iron uptake of radioactive ferrous sulfate was optimal at pH 5.5 using a 2:1 molar ratio of ascorbic acid to iron and a 1-h incubation time. Under these experimental conditions, we studied the effect on iron uptake of adding supernatants from homogenates of different meat sources, soybean protein isolates, egg albumen and bovine serum albumin. Iron uptake was 6.3 +/- 1.7% from meat, which was significantly greater (P < 0.001) than the values of 1.2 +/- 0.3% from soybean protein, 1.3 +/- 0.3% from egg albumen and 0.8 +/- 0.1% from bovine serum albumin. Iron uptake was also significantly higher from digested meat samples than from undigested meat when the protein concentration was equalized. Measurements of iron uptake and protein concentration from fractions obtained after preparative isoelectric focusing of meat and soybean protein extracts showed two peaks of higher protein concentration and iron uptake in meat, apparently not found in soybean protein, that contained the factor(s) responsible for the higher iron uptake by the cells. In view of these observed similarities with iron absorption studies in humans, we conclude that the Caco-2 cell culture system is a useful in vitro model to study food iron availability.