Petrolatum: Barrier repair and antimicrobial responses underlying this "inert" moisturizer
Petrolatum: Barrier repair and antimicrobial responses underlying this "inert" moisturizer
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DOI：
10.1016/j.jaci.2015.08.013
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发表时间：
2016-04-01
影响因子：
14.2
通讯作者：
Guttman-Yassky, Emma
中科院分区：
文献类型：
--
作者：
Czarnowicki, Tali;Malajian, Dana;Guttman-Yassky, Emma
背景：凡士林是一种常见的保湿剂，常用于预防门诊手术后的皮肤感染和特应性皮炎(AD)的维持治疗。方法：对36例健康受试者和13例中度AD患者(平均SCORAD评分39分)进行了RT-PCR、基因芯片、免疫组织化学和免疫荧光检测。结果：抗菌肽显著上调(S100A8/Fold Change[FCH]，13.04；S100A9/FCH，11.28；CCL20/FCH，8.36；CCL20/FCH；PI3[elafin]/Fch，15.40；Lipocalin 2/Fch，6.94，人β-防御素2[DEFB4A]/Fch，4.96；P<；.001)和天然免疫基因(IL6，IL8和IL1B；P<；.01)在凡士林闭塞皮肤中与对照和仅闭塞皮肤相比均有表达。在已知存在屏障和免疫缺陷的“正常”或非皮损的AD皮肤中，应用凡士林还可诱导关键的屏障分化标志物(微丝蛋白和氯化蛋白)的表达，增加角质层厚度，显著减少T细胞的浸润。结论：凡士林对抗菌药物和表皮分化屏障措施具有强大的调节作用。这些数据阐明了凡士林在屏障缺陷状态下的有益分子反应，如AD和术后伤口护理。
Background: Petrolatum is a common moisturizer often used in the prevention of skin infections after ambulatory surgeries and as a maintenance therapy of atopic dermatitis (AD). However, the molecular responses induced by petrolatum in the skin have never been assessed.Objective: We sought to define the cutaneous molecular and structural effects induced by petrolatum.Methods: Thirty-six healthy subjects and 13 patients with moderate AD (mean SCORAD score, 39) were studied by using RT-PCR, gene arrays, immunohistochemistry, and immunofluorescence performed on control skin, petrolatum-occluded skin, and skin occluded with a Finn chamber only.Results: Significant upregulations of antimicrobial peptides (S100A8/fold change [FCH], 13.04; S100A9/FCH, 11.28; CCL20/FCH, 8.36; PI3 [elafin]/FCH, 15.40; lipocalin 2/FCH, 6.94, human beta-defensin 2 [DEFB4A]/FCH, 4.96; P < .001 for all) and innate immune genes (IL6, IL8, and IL1B; P < .01) were observed in petrolatum-occluded skin compared with expression in both control and occluded-only skin. Application of petrolatum also induced expression of key barrier differentiation markers (filaggrin and loricrin), increased stratum corneum thickness, and significantly reduced T-cell infiltrates in the setting of "normal-appearing" or nonlesional AD skin, which is known to harbor barrier and immune defects.Conclusions: Petrolatum robustly modulates antimicrobials and epidermal differentiation barrier measures. These data shed light on the beneficial molecular responses of petrolatum in barrier-defective states, such as AD and postoperative wound care.