A tobacco NtMET1 cDNA encoding a DNA methyltransferase: molecular characterization and abnormal phenotypes of transgenic tobacco plants.
A tobacco NtMET1 cDNA encoding a DNA methyltransferase: molecular characterization and abnormal phenotypes of transgenic tobacco plants.
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编码 DNA 甲基转移酶的烟草 NtMET1 cDNA：转基因烟草植物的分子特征和异常表型。
DOI：
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发表时间：
2000
影响因子：
4.9
通讯作者：
Hiroshi Sano
中科院分区：
文献类型：
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作者：
Yuka Nakano;Nicolas Steward;M. Sekine;Tomonobu Kusano;Hiroshi Sano
从烟草植物中分离到编码DNA甲基转移酶的cDNA，其预测的多肽为1556个氨基酸残基，包含该酶家族中保守的所有基序，相应的基因被命名为NtMET1。 RNA印迹分析表明NtMET1转录物在烟草植物的分裂组织中积累，并且可以在同步分裂的BY2细胞的S期期间检测到它们。原位杂交显示转录本仅位于腋尖分生组织周围活跃增殖的组织中。为了确定生理作用，制备了具有反义构建体的转基因烟草植物并检查了表型。与野生型水平相比，转基因植物的基因组DNA甲基化水平显着降低，并观察到明显的表型变化，包括小叶、短节间和异常花形态。显微镜观察显示转基因植物和野生型植物的叶子结构不同。这些结果表明 NtMET1 在 DNA 复制过程中发挥作用，并且 DNA 甲基化在植物形态发生中起着重要作用。
A cDNA encoding a DNA methyltransferase, with a predicted polypeptide of 1556 amino acid residues containing all motifs conserved in this enzyme family, was isolated from tobacco plants, and the corresponding gene was designated as NtMET1. RNA blot analysis indicated NtMET1 transcripts to accumulate in dividing tissues of tobacco plants, and they could be detected during the S phase in synchronized dividing BY2 cells. In situ hybridization revealed the transcripts to be localized exclusively in actively proliferating tissues around axillary apical meristem. In order to ascertain physiological roles, transgenic tobacco plants that had the antisense construct were made and examined for phenotypes. Methylation levels of genomic DNA from transgenic plants significantly decreased in comparison with wild-type levels, and distinct phenotypic changes including small leaves, short internodes and abnormal flower morphology were noted. Microscopic observation revealed that leaf structure differed between transgenic and wild-type plants. These results suggest that NtMET1 functions during DNA replication, and that DNA methylation plays an important role in plant morphogenesis.
豌豆胞嘧啶 DNA 甲基转移酶 cDNA 的分离、表征和杆状病毒介导的表达。
影响因子：
14.9
作者：
Pradhan,S;Cummings,M;Roberts,RJ;Adams,RL
通讯作者：
Adams,RL