A novel single nucleotide polymorphism in exon 7 of LPL gene and its association with carcass traits and visceral fat deposition in yak (Bos grunniens) steers
A novel single nucleotide polymorphism in exon 7 of LPL gene and its association with carcass traits and visceral fat deposition in yak (Bos grunniens) steers
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LPL基因7号外显子的单核苷酸多态性及其与牦牛胴体性状和内脏脂肪沉积的关系
DOI：
10.1007/s11033-011-0784-4
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发表时间：
2012-01-01
影响因子：
2.8
通讯作者：
Yan, P.
中科院分区：
文献类型：
--
作者：
Ding, X. Z.;Liang, C. N.;Yan, P.
脂蛋白脂酶(LPL)被认为是组织中脂质沉积和代谢的关键酶。该基因被认为是脂类沉积遗传标记的主要候选基因。因此，本研究采用聚合酶链式反应-单链构象多态性分析和DNA测序技术，对5个牦牛品种的398个个体进行了LPL基因的多态性分析及其与屠体性状和内脏脂肪含量的关联分析。发现了一种新的核苷酸多态-C→T(Nt19913)，它位于Lp1基因编码区的第7外显子，其替换导致了氨基酸由Phe到Ser的替换。在扩增产物中检测到2个等位基因(A、B)和3个等位基因AA、AB、BB。天祝白牦牛(WY)、甘南牦牛(GY)、青海高原牦牛(PY)、新疆牦牛(XY)和大同牦牛(DY)等位基因A的频率分别为0.7928、0.7421、0.7357、0.6900和0.7083。5个牦牛群体的SNP基因座均符合Hardy-Weinberg平衡(P>；P>；0.05)。LPL基因的多态性与屠体性状和脂肪沉积有关。最小二乘分析表明，添加不同处理对活重(LW)、平均日增重(ADG)和屠体重(P<；)均有显著影响(P<；0.01)。在25~36个月龄，BB基因携带者的眼肌面积和内脏脂肪重量(占体重的百分比)的平均值低于AA和AB基因携带者(P<；P<0.05)。结果表明，LPL基因是影响牦牛屠体性状和脂肪沉积的重要候选基因。
Lipoprotein lipase (LPL) is considered as a key enzyme in the lipid deposition and metabolism in tissues. It is assumed to be a major candidate gene for genetic markers in lipid deposition. Therefore, the polymorphisms of the LPL gene and associations with carcass traits and viscera fat content were examined in 398 individuals from five yak (Bos grunniens) breeds using PCR–SSCP analysis and DNA sequencing. A novel nucleotide polymorphism (SNP)-C→T (nt19913) was identified located in exon 7 in the coding region of the LPL gene, which replacement was responsible for a Phe-to-Ser substitution at amino acid. Two alleles (A and B) and three genotypes designed as AA, AB and BB were detected in the PCR products. The frequencies of allele A were 0.7928, 0.7421, 0.7357, 0.6900 and 0.7083 for Tianzhu white yak (WY), Gannan yak (GY), Qinghai-Plateau yak (PY), Xinjiang yak (XY) and Datong yak (DY), respectively. The SNP loci was in Hardy–Weinberg equilibrium in five yak populations (P> 0.05). Polymorphism of LPL gene was shown to be associated with carcass traits and lipid deposition. Least squares analysis revealed that there was a significant effect on live-weight (LW) (P< 0.01), average daily weight gain (ADG) and carcass weight (P< 0.05). Individuals with genotype BB had lower mean values than those with genotype AA and AB for loin eye area and viscera fat weight (% of LW) in 25–36 months (P< 0.05). The results indicated that LPL gene is a strong candidate gene that affects carcass traits and fat deposition in yak.