Bmp4 is required for the generation of primordial germ cells in the mouse embryo
Bmp4 is required for the generation of primordial germ cells in the mouse embryo
复制标题
DOI：
10.1101/gad.13.4.424
复制
发表时间：
1999-02-15
影响因子：
10.5
通讯作者：
Hogan, BLM
中科院分区：
文献类型：
--
作者：
Lawson, KA;Dunn, NR;Hogan, BLM
在许多生物体中，原始生殖细胞（PGCs）的分配是由储存在卵中的母体因子的遗传决定的。然而，在哺乳动物中，在原肠胚形成周围需要诱导细胞相互作用以建立生殖系。在这里，我们表明，Bmp 4纯合无效胚胎不含PGCs。它们也缺乏尿囊，尿囊是一种胚外中胚层组织，像PGCs一样，来自近端上胚层的前体。杂合子的原始生殖细胞比正常人少，这是由于原始群体的规模减少，而不是对其随后的扩张产生影响。Bmp 4（lacZneo）胚胎中β-半乳糖苷酶活性的分析揭示，在原肠胚形成之前，Bmp 4在胚外胚层中表达。后来，Bmp 4在胚外中胚层中表达，但在PGCs中不表达。嵌合体分析表明，Bmp 4在胚外外胚层中的表达调节尿囊和原始生殖细胞前体的形成，以及PGCs的建立群体的大小。因此，小鼠生殖系的起始依赖于来自先前分离的胚外滋养外胚层谱系的分泌信号。
In many organisms the allocation of primordial germ cells (PGCs) is determined by the inheritance of maternal factors deposited in the egg. However, in mammals, inductive cell interactions are required around gastrulation to establish the germ line. Here, we show that Bmp4 homozygous null embryos contain no PGCs. They also lack an allantois, an extraembryonic mesodermal tissue derived, like the PGCs, from precursors in the proximal epiblast. Heterozygotes have fewer PGCs than normal, due to a reduction in the size of the founding population and not to an effect on its subsequent expansion. Analysis of beta-galactosidase activity in Bmp4(lacZneo) embryos reveals that prior to gastrulation, Bmp4 is expressed in the extraembryonic ectoderm. Later, Bmp4 is expressed in the extraembryonic mesoderm, but not in PGCs. Chimera analysis indicates that it is the Bmp4 expression in the extraembryonic ectoderm that regulates the formation of allantois and primordial germ cell precursors, and the size of the founding population of PGCs. The initiation of the germ line in the mouse therefore depends on a secreted signal from the previously segregated, extraembryonic, trophectoderm lineage.