Elucidating the origin of chromosomal aberrations in IVF embryos by preimplantation genetic analysis
Elucidating the origin of chromosomal aberrations in IVF embryos by preimplantation genetic analysis
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DOI：
10.1016/j.mce.2007.11.009
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发表时间：
2008-01-30
影响因子：
4.1
通讯作者：
Ben-Yosef, Dalit
中科院分区：
文献类型：
--
作者：
Frumkin, Tsvia;Malcov, Mira;Ben-Yosef, Dalit
植入前遗传筛查（PGS）已被提出作为一种方法，以提高成功率的患者反复IVF失败。这种方法是基于这样的假设，即这种失败是非整倍体胚胎的结果。有人认为，从植入前胚胎中取出的卵裂球的FISH分析代表了整个胚胎的染色体构成。然而，目前尚不清楚它是否也代表了植入胚胎的染色体构成。在第3天被指定为“非整倍体”的胚胎在第5天的PGS再分析可以证明染色体畸变的嵌合率高。这些嵌合体胚胎中的一些能够通过“自我校正”发育成正常胚胎。然而，其他人可能会积累额外的染色体异常。因此，可以得出结论，植入前胚胎的染色体组成可能在早期卵裂过程中演变。减数分裂和合子后有丝分裂错误可能是这些染色体畸变的原因。本文通过对不同时期胚胎染色体组成的研究，阐明了植入前胚胎发育过程中染色体变化的起源。(c)2007爱思唯尔爱尔兰有限公司保留所有权利。
Preimplantation genetic screening (PGS) has been proposed as a method for improving success rates in patients with repeated IVF failures. This approach is based on the hypothesis that such failures are the result of aneuploid embryos. It has been suggested that FISH analysis of blastomeres removed from preimplantation embryos represent the chromosomal constitution of the entire embryo. However, it is not yet clear whether it also represents the chromosomal constitution of the implanted embryo. PGS reanalysis on day 5 of embryos designated as "aneuploid" on day 3 may demonstrate a high rate of mosaicism for chromosomal aberration. Some of these mosaic embryos are capable of developing into normal embryos by "self-correction". Others, however, may accumulate additional chromosomal anomalies. It is therefore concluded that the chromosomal constitution of a preimplantation embryo may evolve during early cleavages. Meiotic and post zygotic mitotic errors may account for these chromosomal aberrations. This review will focus on elucidating the origin of chromosomal changes during preimplantation embryo development by studying their chromosomal constitution at different stages. (c) 2007 Elsevier Ireland Ltd. All rights reserved.