MODULATION OF FLUID PUMPING IN ISOLATED BOVINE MESENTERIC LYMPHATICS BY A THROMBOXANE ENDOPEROXIDE ANALOG
MODULATION OF FLUID PUMPING IN ISOLATED BOVINE MESENTERIC LYMPHATICS BY A THROMBOXANE ENDOPEROXIDE ANALOG
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DOI：
10.1016/0090-6980(88)90105-0
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发表时间：
1988-07-01
影响因子：
2.9
通讯作者：
JOHNSTON, MG
中科院分区：
文献类型：
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作者：
ELIAS, RM;JOHNSTON, MG
已知血栓烷/内过氧化物类似物（化合物U46619）刺激静止牛淋巴管中的阶段性和紧张性收缩，并增强自发活动血管中的收缩活性。为了确定这些影响如何与流体推进的变化，通过动脉，我们已经评估了U46619的影响的能力，分离的牛肠系膜动脉泵送液体在体外。牛淋巴节段（最长8 cm，最少4个瓣膜）两端插管，并从储液器输入液体。通过管腔内压力调节通过血管的流量。平均而言，跨壁压的变化高达8厘米水柱导致增强泵送，压力高于这个水平的抑郁症的流量。U46619（添加到储药器中）的主要作用是抑制泵送; 10-7和10- 9 M在所有测试的跨壁压下降低流量; 10- 8 M具有双重作用，在低跨壁压下轻微抑制流量，在较高压力下增强流量。这些结果表明，血栓素可以刺激或抑制淋巴泵取决于药物的浓度和跨壁压力施加到血管。这些效应可能与其诱导管腔直径和收缩频率和收缩力发生可变变化的能力有关。
A thromboxane/endoperoxide analogue (compound U46619) is known to stimulate phasic and tonic contractions in quiescent bovine lymphatic vessels and enhance contractile activity in spontaneously active vessels. In order to determine how these effects relate to changes in fluid propulsion by the lymphatics, we have assessed the effects of U46619 on the ability of isolated bovine mesenteric lymphatics to pump fluid in vitro. Bovine lymphatic segments (up to 8 cm in length with a minimum of 4 valves) were cannulated at both ends and fluid input provided from a reservoir. Flow through the vessels was regulated by intraluminal pressures. On average, changes in transmural pressures up to 8 cm H2O resulted in enhanced pumping; pressures above this level depressed flow. The dominant effect of U46619 (added to the reservoir) was to depress pumping; 10-7 and 10-9M decreased flow at all transmural pressures tested; 10-8M had a dual effect, slightly inhibiting flow at low transmural pressures and enhancing flow at higher pressures. These results suggest that thromboxane may stimulate or inhibit lymphatic pumping depending on the concentration of the agent and the transmural pressure applied to the vessel. These effects may relate to its ability to induce variable changes in luminal diameter and frequency and force of contractions.