Structure of the bacterial flagellar rotor MS-ring: a minimum inventory/maximum diversity system
Structure of the bacterial flagellar rotor MS-ring: a minimum inventory/maximum diversity system
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DOI：
10.1101/718072
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发表时间：
2019-07
期刊：
影响因子：
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通讯作者：
Steven Johnson;Y. Fong;J. Deme;E. Furlong;L. Kuhlen;S. Lea
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文献类型：
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作者：
Steven Johnson;Y. Fong;J. Deme;E. Furlong;L. Kuhlen;S. Lea
细菌鞭毛是一种复杂的、自组装的纳米机器，赋予细胞运动性。尽管物种间存在很大差异，但所有鞭毛最终都是由螺旋推进器连接到嵌入内膜的马达上构建的。电机由一系列定子单元组成，定子单元围绕由两个环复合体（MS环和C环）组成的中心转子。尽管有许多研究，高分辨率的结构信息仍然是完全缺乏的MS环的转子，并提出了不匹配的化学计量之间的两个环长期以来提供了一个来源的混乱的领域。我们在这里提出的沙门氏菌MS环的结构，揭示了前所未有的水平间和链内对称性的变化，提供了一个结构解释的MS环的功能作为一个复杂的和优雅的界面之间的两个主要功能的鞭毛，蛋白质分泌和旋转的能力。
The bacterial flagellum is a complex, self-assembling, nanomachine that confers motility on the cell. Despite great variation across species, all flagella are ultimately constructed from a helical propellor attached to a motor embedded in the inner membrane. The motor consists of a series of stator units surrounding a central rotor made up of two ring complexes, the MS-ring and the C-ring. Despite many studies, high resolution structural information is still completely lacking for the MS-ring of the rotor, and proposed mismatches in stoichiometry between the two rings have long provided a source of confusion for the field. We here present structures of the Salmonella MS-ring, revealing an unprecedented level of inter- and intra-chain symmetry variation that provides a structural explanation for the ability of the MS-ring to function as a complex and elegant interface between the two main functions of the flagellum, protein secretion and rotation.