Asna1/TRC40-mediated membrane insertion of tail-anchored proteins
Asna1/TRC40-mediated membrane insertion of tail-anchored proteins
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DOI：
10.1242/jcs.055970
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发表时间：
2010-05-01
影响因子：
4
通讯作者：
Dobberstein, Bernhard
中科院分区：
文献类型：
--
作者：
Favaloro, Vincenzo;Vilardi, Fabio;Dobberstein, Bernhard
尾部锚定(Tail-Anchored，TA)蛋白在翻译后插入内质网(ER)的膜，并通过其C端跨膜结构域跨膜。我们从重组Asna 1/TA蛋白复合体和内质网衍生的膜中重组了TA蛋白的膜插入。我们的数据表明Asna 1可以介导RAMP4和Sec61的膜插入。在没有其他胞浆蛋白参与的情况下，这一机制依赖于内质网膜上是否存在ATP或ADP以及一个对蛋白酶敏感的受体。相比之下，细胞色素b5的膜插入可以独立于Asna 1和核苷酸进行。
Tail-anchored (TA) proteins insert post-translationally into the membrane of the endoplasmic reticulum (ER) and span the membrane by their C-terminal transmembrane domain. We have reconstituted membrane insertion of TA proteins from recombinant Asna1/TA protein complexes and ER-derived membranes. Our data show that Asna1 can mediate membrane insertion of RAMP4 and Sec61. without the participation of other cytosolic proteins by a mechanism that depends on the presence of ATP or ADP and a protease-sensitive receptor in the ER membrane. By contrast, membrane insertion of cytochrome b5 can proceed independently of Asna1 and nucleotides.