Identification of genes up-regulated in response to Cd exposure, in Brassica juncea L
Identification of genes up-regulated in response to Cd exposure, in Brassica juncea L
复制标题
DOI：
10.1016/j.gene.2005.07.037
复制
发表时间：
2005-12-19
期刊：
影响因子：
3.5
通讯作者：
Chai, TY
中科院分区：
文献类型：
--
作者：
Minglin, L;Zhang, YX;Chai, TY
利用荧光mRNA差异显示（DD）技术研究了重金属积累植物芥菜（Brassica juncea）对Cd胁迫的转录调控。共鉴定出154条DD带，其中15条和13条分别从叶片和根中克隆到。许多基因被证实有2-5倍的表达增加，在根和叶48小时后，镉暴露（类似于22.4 ppm）。然而，一些分离的基因，例如，DD 2、DD 21、DD 22的mRNA表达模式相反。测序结果表明，Cd诱导表达上调的基因共涉及19个基因，其中18个基因与拟南芥基因序列完全一致，并确定了15个基因的功能，包括生长素响应基因GH 3、ARF样小GTP酶/ARF、ARD/ARD '、APS还原酶、Nop、过氧化氢酶、锌指蛋白等（C3 HC 4型环指）、二酰基甘油激酶和卤酸脱卤酶样水解酶家族。三个cDNA对应于预测的膜蛋白（KOG 3491）或核糖体相关的膜蛋白RAMP 4。另一个克隆DD 26与GenBank数据库中的任何翻译序列都没有显示出显著的同一性，这表明它可能编码未鉴定的蛋白质，或者对应于mRNA分子的非翻译、非保守区域。这些Cd响应性上调基因大多也受非生物或生物胁迫的调节，例如，脱水、寒冷、高盐、生长素、高温和感染。本研究导致增加了解基因和/或参与重金属抗性和积累的植物的生化途径。(c)2005年由Elsevier B. V.出版
In this paper, the fluorescent mRNA differential display (DD) technique was applied to analyze transcriptional regulation in response to Cd treatment in a heavy-metal accumulator, Brassica juncea. 154 DD bands were identified, of which fragments corresponding to 15 and 13 cDNAs were successfully cloned from leaves and roots, respectively. Many of the genes were confirmed to have a 2-5 fold increase in expression in both roots and leaves after 48 h Cd exposure (similar to 22.4 ppm). However, several isolated genes, e.g., DD2, DD21, DD22, showed a reversed mRNA expression pattern. Sequencing revealed those Cd-induced up-regulated genes displayed mRNAs corresponding to 19 different genes, 18 of which had a clear identity to Arabidopsis thaliana sequences and a putative function was assigned to 15 of them, including the auxin-responsive GH3, ARF-like small GTPases/ARFs, ARD/ARD', APS reductase, Nop, catalase, zinc finger (C3HC4-type RING finger), diacylglycerol kinase, and haloacid dehalogenase-like hydrolase families. Three cDNAs corresponded to predicted membrane proteins (KOG3491) or a ribosome-associated membrane protein RAMP4. One other clone, DD26, did not show significant identities to any translated sequence in the GenBank database, suggesting it may either encode unidentified proteins, or correspond to un-translated, non-conserved regions of mRNA molecules. These Cd-responsive up-regulated genes are mostly also regulated by abiotic or biotic stresses, e.g., dehydration, chilling, high salt, auxin, heat and infection, in other plants. The present study leads to an increased understanding of genes and/or the biochemical pathways involved in heavy-metal resistance and accumulation in plants. (c) 2005 Published by Elsevier B.V.