Polyphenols in the Fermentation Liquid ofDendrobium candidumRelieve Intestinal Inflammation in Zebrafish Through the Intestinal Microbiome-Mediated Immune Response
Polyphenols in the Fermentation Liquid ofDendrobium candidumRelieve Intestinal Inflammation in Zebrafish Through the Intestinal Microbiome-Mediated Immune Response
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DOI：
10.3389/fimmu.2020.01542
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发表时间：
2020-07-17
影响因子：
7.3
通讯作者：
Zhang, Jiachao
中科院分区：
文献类型：
--
作者：
Gong, Xiaoyue;Jiang, Shuaiming;Zhang, Jiachao
铁皮石斛的研究进展。主要分布于热带地区的念珠菌（Candidum），对其功能性多糖的研究较多；解析：选d。念珠菌多酚，其化学成分可以通过发酵来改善，受到的关注有限，特别是在体内模型中，它不可避免地涉及与肠道微生物的相互作用。为了解决这一挑战，应用了宏基因组和代谢组学技术，并测定了免疫因子和粘膜屏障相关蛋白，以揭示发酵dd的作用。念珠菌多酚（FDC）对恶唑酮诱导斑马鱼肠道炎症的影响。结果表明，发酵显著改变了d的化学成分。念珠菌表明，FDC显著提高了肠道免疫指数。FDC干预第21天，乳酸菌、粪杆菌、瘤胃杆菌的丰度增加，而雪瓦氏菌属、嗜土杆菌属、胃链球菌科、分枝杆菌属的丰度下降。同时，FDC显著增加了肠道短链脂肪酸（SCFAs）。此外，基于多组学的网络分析表明，摄入FDC可导致肠道菌群和肠道代谢物发生变化，从而增强宿主免疫功能。上述结果提示，FDC可通过调节肠道菌群及其代谢物，治疗肠道炎症，调节宿主免疫系统，从而改善肠道健康。本研究提高了我们对d的利用的认识。念珠菌多酚，为发酵dd的影响提供了新的证据。念珠菌宿主健康。
Previous studies ofDendrobium candidum(D. candidum), which is mainly distributed in tropical areas, have mainly focused on its functional polysaccharide; the effects ofD. candidumpolyphenols, the chemical composition of which may be improved by fermentation, have received limited attention, especially inin vivomodels, which inevitably involve interactions with intestinal microorganisms. To address this challenge, metagenomic and metabolomic techniques, were applied, and immune factors and mucosal barrier-related proteins were determined to reveal the effects of fermentedD. candidumpolyphenols (FDC) on intestinal inflammation induced by oxazolone in zebrafish. The results showed that fermentation significantly changed the chemical composition ofD. candidumand that FDC significantly improved the intestinal immune index. After the 21st day of FDC intervention, the abundance ofLactobacillus, Faecalibacterium, andRummeliibacillusincreased, but the abundance of the generaShewanella, Geodermatophilus, Peptostreptococcaceae, andMycobacteriumdecreased. At the same time, FDC significantly increased intestinal short-chain fatty acids (SCFAs). In addition, network analysis based on multi-omics indicated that FDC intake leads to changes in intestinal microbiota and intestinal metabolites, resulting in enhanced host immune function. These results indicate that FDC can improve intestinal health by regulating the intestinal microbiota and its metabolites to treat intestinal inflammation and regulate the host immune system. The present research improved our understanding of the utilization ofD. candidumpolyphenols and provided new evidence for the impacts of fermentedD. candidumon host health.