Sphingosine-1-phosphate regulates the expression of adherens junction protein E-cadherin and enhances intestinal epithelial cell barrier function.
Sphingosine-1-phosphate regulates the expression of adherens junction protein E-cadherin and enhances intestinal epithelial cell barrier function.
复制标题
DOI：
10.1007/s10620-010-1421-0
复制
发表时间：
2011-05
影响因子：
3.1
通讯作者：
Turner, Douglas J.
中科院分区：
文献类型：
--
作者：
Greenspon, Jose;Li, Ruiyun;Xiao, Lan;Rao, Jaladanki N.;Sun, Rex;Strauch, Eric D.;Shea-Donohue, Terez;Wang, Jian-Ying;Turner, Douglas J.
肠道屏障通透性的调节对维持正常的肠道生理功能具有重要意义。鞘氨醇-1-磷酸(S1P)在增强多种非肠道组织的屏障功能中起着关键作用。目前的研究确定了S1P是否通过改变黏附连接中的重要蛋白质E-钙粘蛋白的表达来调节肠上皮屏障的功能。使用标准技术对培养的分化的IECs(IEC-CDx2L1系)进行研究。S1P处理显著增加肠上皮细胞(IECs)中E-钙粘蛋白的蛋白和mRNA水平，并导致E-钙粘蛋白强烈定位于细胞-细胞边界。S1P还改善了屏障功能，表现为14C-甘露醇细胞旁通透性降低和体外跨皮细胞电阻(TEER)增加。这些结果表明，S1P增加了E-钙粘附素在细胞量和细胞-细胞连接处的水平，并导致培养的肠上皮细胞屏障完整性的改善。
The regulation of intestinal barrier permeability is important in the maintenance of normal intestinal physiology. Sphingosine-1-phosphate (S1P) has been shown to play a pivotal role in enhancing barrier function in several non-intestinal tissues. The current study determined whether S1P regulated function of the intestinal epithelial barrier by altering expression of E-cadherin, an important protein in adherens junctions. Studies were performed upon cultured differentiated IECs (IEC-Cdx2L1 line) using standard techniques. S1P treatment significantly increased levels of E-cadherin protein and mRNA in intestinal epithelial cells (IECs) and also led to E-cadherin localizing strongly to the cell–cell border. S1P also improved the barrier function as indicated by a decrease in 14C-mannitol paracellular permeability and an increase in transepithelial electrical resistance (TEER) in vitro. These results indicate that S1P increases levels of E-cadherin, both in cellular amounts and at the cell–cell junctions, and leads to improved barrier integrity in cultured intestinal epithelial cells.
登录
查看更多内容
影响因子：
3.1
作者：
Carneiro, BA;Lima, IPF;Lima, AAM
通讯作者：
Lima, AAM
影响因子：
20.3
作者：
Corada, M;Liao, F;Dejana, E
通讯作者：
Dejana, E
DOI：
10.1152/ajpgi.00407.2004
发表时间：
2005-06-01
影响因子：
4.5
作者：
Guo, X;Rao, JN;Wang, JY
通讯作者：
Wang, JY
影响因子：
7.8
作者：
Angres, B;Barth, A;Nelson, WJ
通讯作者：
Nelson, WJ
通过表达突变型紧密连接膜蛋白，细胞旁通透性和跨上皮电阻的功能性分离以及顶端-基底外侧膜内扩散屏障的破坏。
影响因子：
7.8
作者：
Balda, M S;Whitney, J A;Flores, C;Gonzalez, S;Cereijido, M;Matter, K
通讯作者：
Matter, K