Subunit composition of rat liver glutathione S-transferases.
Subunit composition of rat liver glutathione S-transferases.
复制标题
大鼠肝脏谷胱甘肽 S-转移酶的亚基组成。
DOI：
10.1016/0006-291x(82)90851-8
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发表时间：
1982
影响因子：
3.1
通讯作者：
Reddy,CC
中科院分区：
文献类型：
--
作者：
Tu,CP;Weiss,MJ;Reddy,CC
质粒pGTR 112含有大鼠肝谷胱甘肽S-转移酶亚基之一的部分编码序列。我们已经使用固定化的pGTR 112 DNA在增加杂交严格性的条件下选择互补和同源的肝脏多聚（A）-RNA。选择的poly（A）-RNA的每个部分通过体外翻译和免疫沉淀进行分析。用NaDodSO_4聚丙烯酰胺凝胶电泳分离了四种不同的抗大鼠肝谷胱甘肽S-转移酶抗血清沉淀的多肽。根据它们的poly（A）-RNA与pGTR 112 DNA的序列同源性，它们被分成两对。纯化的大鼠肝谷胱甘肽S-转移酶可在梯度NaDodSO_4聚丙烯酰胺凝胶上分离成四种多肽。大鼠肝谷胱甘肽S-转移酶的四种不同亚基应具有十种不同二元组合的同工酶。
The plasmid pGTR112 contains partial coding sequences for one of the rat liver glutathione S-transferase subunits. We have used immobilized pGTR112 DNA to select for complementary and homologous liver poly(A)-RNAs under conditions of increasing stringency for hybridization. Each fraction of selected poly(A)-RNAs was assayed byin vitrotranslation followed by immunoprecipitation. A total of four distinct polypeptides precipitated by antiserum against rat liver glutathione S-transferases were resolved by NaDodSO4polyacrylamide gel electrophoresis. They are separated into two pairs according to the sequence homology of their poly(A)-RNAs with the pGTR112 DNA. Purified rat liver glutathione S-transferases can be resolved on gradient NaDodSO4polyacrylamide gels into four polypeptides. There should be ten isozymes of different binary combinations from four distinct subunits for the rat liver glutathione S-transferases.