Cytogenetic abnormalities of the descendants of permanent residents of heavily contaminated East Kazakhstan
Cytogenetic abnormalities of the descendants of permanent residents of heavily contaminated East Kazakhstan
复制标题
严重污染的东哈萨克斯坦永久居民后代的细胞遗传学异常
DOI：
10.1007/s00411-017-0717-2
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Inoue Ken
中科院分区：
文献类型：
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作者：
Chaizhunusova Nailya;Madiyeva Madina;Tanaka Kimio;Hoshi Masaharu;Kawano Noriyuki;Noso Yoshihiro;Takeichi Nobuo;Rakhypbekov Tolebay;Urazalina Nailya;Dovgal Galina;Rymbaeva Tamara;Tokanova Sholpan;Beisengazina Meruert;Kembayeva Kulypash;Inoue Ken
在塞米巴拉金斯克核试验场进行了400多次核爆炸试验，释放了大量放射性物质。在试验场关闭约25年后，对在南部核试验场进行的活动的长期后果以及出现的任何遗传影响仍然没有进行充分的研究。生活在SNTS附近村庄的人口被认为受到了严重的内外辐射照射。本研究旨在对SNTS附近的居民及其第一代（F1）和第二代（F2）子女进行评估和全面的细胞遗传学分析。该研究包括居住在前SNTS覆盖地区的东哈萨克斯坦地区居民。为了评估核试验的遗传影响，使用传统的姬姆萨和荧光原位杂交方法对多隆和萨尔扎尔那些村庄的115个F1和F2后代的淋巴细胞进行了全面的染色体分析，这些村庄受到严重污染。受试者的父母长期居住在那些村庄。在这些居民中发现的易位等稳定型染色体畸变的数量高于对照区Kokpecty的80名居民，这表明辐射可能对受照者产生生物效应。
More than 400 nuclear explosion tests were conducted at the Semipalatinsk Nuclear Test Site (SNTS) and significant radioactive substances were released. The long-term consequences of the activities at the SNTS and the appearance of any hereditary effects remain insufficiently studied about 25 years after the test site was closed. The population living in villages near the SNTS are considered to have been heavily exposed to external and internal radiation. This study aims to perform an assessment and comprehensive cytogenetic analysis of the inhabitants living near the SNTS, and their first-(F1) and second-(F2) generation children. Residents of the East Kazakhstan region living in the area covered by the former SNTS were included in the study. To evaluate the hereditary effects of nuclear testing, comprehensive chromosome analyses were performed in lymphocytes using conventional Giemsa and fluorescent in situ hybridization methods in 115 F1 and F2 descendants in the villages of Dolon and Sarzhal, which were heavily contaminated. The parents of the subjects had permanently lived in the villages. A higher number of stable-type chromosome aberrations such as translocations was found in these residents than in 80 residents of the control area, Kokpecty, which indicates the possibility that radiation had biological effects on the exposed subjects.