Bubble-mediated transport of benthic microorganisms into the water column: Identification of methanotrophs and implication of seepage intensity on transport efficiency
Bubble-mediated transport of benthic microorganisms into the water column: Identification of methanotrophs and implication of seepage intensity on transport efficiency
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DOI：
10.1038/s41598-020-61446-9
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发表时间：
2020-03
影响因子：
4.6
通讯作者：
S. Jordan;T. Treude;I. Leifer;René Janßen;Johannes Werner;H. Schulz-Vogt;O. Schmale
中科院分区：
文献类型：
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作者：
S. Jordan;T. Treude;I. Leifer;René Janßen;Johannes Werner;H. Schulz-Vogt;O. Schmale
底栖微生物进入水柱可能影响海洋地球化学循环和浮游食物网结构。在本研究中，六个气体释放喷口的网站在煤油点渗漏领域（加州）进行了调查，并记录了从海底释放的气泡从沉积物中的微生物进入水柱的位错。结果表明，甲烷氧化菌的传输效率与气体体积流量有关，在气体体积流量为0.07 mLgass−1时，甲烷氧化菌的传输速率最高，为22.7 × 103 cells mLgas− 1;在气体体积流量为2.2 mLgass−1时，甲烷氧化菌的传输速率最低，为0.2 × 103 cells mLgas− 1。一个简单的预算方法表明，这种气泡介导的运输有可能保持相关部分的水柱甲烷氧化菌在渗漏场。气泡介导的底栖和浮游环境之间的联系得到遗传分析的进一步支持，这表明甲烷氧化菌的家庭Methylomonaceae和石油降解细菌的属Cycloclasticus从沉积物运输到水柱。这些研究结果表明，气泡介导的微生物运输影响的浮游微生物丰度和社区组成的气体释放渗漏网站。
Benthic microorganisms transported into the water column potentially influence biogeochemical cycles and the pelagic food web structure. In the present study six gas-releasing vent sites in the Coal Oil Point seep field (California) were investigated, and the dislocation of microorganisms from the sediment into the water column via gas bubbles released from the seabed was documented. It was found that the methanotrophs transport efficiency was dependent on the volumetric gas flow, with the highest transport rate of 22.7 × 103cells mLgas−1at a volumetric gas flow of 0.07 mLgass−1, and the lowest rate of 0.2 × 103cells mLgas−1at a gas flow of 2.2 mLgass−1. A simple budget approach showed that this bubble-mediated transport has the potential to maintain a relevant part of the water-column methanotrophs in the seep field. The bubble-mediated link between the benthic and pelagic environment was further supported by genetic analyses, indicating a transportation of methanotrophs of the familyMethylomonaceaeand oil degrading bacteria of the genusCycloclasticusfrom the sediment into the water column. These findings demonstrate that the bubble-mediated transport of microorganisms influences the pelagic microbial abundance and community composition at gas-releasing seep sites.