MIGRATORY BEHAVIOR OF CARDIAC CUSHION TISSUE-CELLS IN A COLLAGEN-LATTICE CULTURE SYSTEM
MIGRATORY BEHAVIOR OF CARDIAC CUSHION TISSUE-CELLS IN A COLLAGEN-LATTICE CULTURE SYSTEM
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DOI：
10.1016/0012-1606(82)90030-6
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发表时间：
1982-01-01
影响因子：
2.7
通讯作者：
MARKWALD, RR
中科院分区：
文献类型：
--
作者：
BERNANKE, DH;MARKWALD, RR
本文设计了一种培养体系，用于研究影响心肌垫组织细胞迁移的因素。将离体鸡房室管垫的外植体置于水合胶原晶格上。细胞从内膜的外植体中生长出来，并穿过胶原蛋白网格的表面。在进一步孵育期间，间充质型细胞从表面群体接种到下面的胶原基质中。这些细胞在形态上类似于间充质垫组织细胞，其来源于内皮细胞，并迁移到胚胎心脏中的心脏胶质基质中并穿过心脏胶质基质。在培养系统中观察到的事件模仿发生在发育中的鸡房室垫。
A culture system was devised for the study of factors which influence the migration of cardiac cushion tissue cells. Explants of isolated chick atrioventricular canal cushions were placed on hydrated collagen lattices. Cells grew out of the explants from the endocardium and across the surface of the collagen lattices. During further incubation, mesenchyme-type cells seeded from the surface population into the underlying collagen matrix. These cells were morphologically similar to the mesenchymal cushion tissue cells which are derived from the endocardium and which migrate into and through the cardiac jelly matrix in the embryonic heart. The events observed in the culture system mimicked those occurring in the developing chick atrioventricular cushion.