Construction and characterization of an infectious clone generated from Chikungunya virus SL11131 strain
Construction and characterization of an infectious clone generated from Chikungunya virus SL11131 strain
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基孔肯雅病毒 SL11131 株感染性克隆的构建和表征
DOI：
10.1016/j.virol.2020.09.006
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Nakano T
中科院分区：
文献类型：
--
作者：
Suzuki Y;Tanaka A;Maeda Y;Emi A;Fujioka Y;Sakaguchi S;SG Vasudevan;Kobayashi T ;CK Lim;Takasaki T;H Wu;Nakano T
基孔肯雅病毒(CHIKV)是一种由蚊子传播的RNA病毒，可在人类中引起基孔肯雅热。在这项研究中，我们产生了两个基于DNA的CHIKV感染性克隆，这些克隆来自印度洋的SL11131株和罗斯的原型株。当评估传染性CHIKV在不同细胞系中的复制能力时，发现SL11131株在白纹伊蚊C6/36细胞中的复制效率高于Ross株，而SL11131株在BHK-21衍生细胞系BHK-DRV中的复制能力有限。SL11131和Ross之间的嵌合CHIKV感染实验表明，SL11131在C6/36和BHK-DRV细胞中的这种不同复制活性分别由结构基因和非结构基因决定。因此，本研究建立的感染性克隆将为研究新近流行的CHIKV毒株的病毒学特征提供有用的工具，并有助于开展CHIKV感染的有效防治。
Chikungunya virus (CHIKV) is a mosquito-borne RNA virus that causes Chikungunya fever in humans. In this study, we generated two DNA-based CHIKV infectious clones derived from an Indian Ocean Lineage SL11131 strain and a prototype Ross strain. When the replication capabilities of the infectious CHIKV in various cell lines were evaluated, the SL11131 strain was found to replicate more efficiently than the Ross strain inAedes albopictusC6/36 cells, whereas SL11131 underwent limited replication in a BHK-21-derivative cell line named BHK-DRV. Infection experiments using chimeric CHIKV between SL11131 and Ross revealed that these different replication activities of SL11131 in C6/36 and BHK-DRV cells were determined by structural and nonstructural genes, respectively. Therefore, the infectious clones created in this study will be a useful tool for investigating the virological features of a recent epidemic strain of CHIKV and benefit the development of effective prevention and treatment of CHIKV infection.