Digital Museum of Retinal Ganglion Cells with Dense Anatomy and Physiology
Digital Museum of Retinal Ganglion Cells with Dense Anatomy and Physiology
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DOI：
10.1016/j.cell.2018.04.040
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发表时间：
2018-05-17
期刊：
影响因子：
64.5
通讯作者：
Seung, H. Sebastian
中科院分区：
文献类型：
--
作者：
Bae, J. Alexander;Mu, Shang;Seung, H. Sebastian
当3D电子显微镜和钙成像用于研究神经回路的结构和功能时，所产生的数据集对可视化和解释提出了新的挑战。在这里，我们提出了一种新的数字资源，它包含了来自一片小鼠视网膜的近400个神经节细胞。一个在线“博物馆”提供了每个细胞解剖结构的3D交互式视图，以及其视觉反应的图表。该资源揭示了视网膜的内丛状层的两个方面：一个乔木隔离原则的结构沿着光轴和密度守恒原则的结构在切平面上的管理。结构与视觉功能有关;平均而言，在神经节细胞胞体层附近具有乔木的神经节细胞在其视觉反应中更持久。我们的方法可能适用于神经系统其他部分的神经元解剖学和生理学的密集地图。
When 3D electron microscopy and calcium imaging are used to investigate the structure and function of neural circuits, the resulting datasets pose new challenges of visualization and interpretation. Here, we present a new kind of digital resource that encompasses almost 400 ganglion cells from a single patch of mouse retina. An online "museum'' provides a 3D interactive view of each cell's anatomy, as well as graphs of its visual responses. The resource reveals two aspects of the retina's inner plexiform layer: an arbor segregation principle governing structure along the light axis and a density conservation principle governing structure in the tangential plane. Structure is related to visual function; ganglion cells with arbors near the layer of ganglion cell somas are more sustained in their visual responses on average. Our methods are potentially applicable to dense maps of neuronal anatomy and physiology in other parts of the nervous system.