Microsatellites as DNA markers in cultivated peanut (Arachis hypogaea L.).
Microsatellites as DNA markers in cultivated peanut (Arachis hypogaea L.).
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微卫星作为培养花生中的DNA标记（Arachis hypogaea L.）。
DOI：
10.1186/1471-2229-3-3
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发表时间：
2003-04-24
影响因子：
5.3
通讯作者：
Prakash, C S
中科院分区：
文献类型：
--
作者：
He, Guohao;Meng, Ronghua;Newman, Melanie;Gao, Guoqing;Pittman, Roy N;Prakash, C S
由于花生遗传多样性DNA标记的缺乏，花生基因组研究一直落后于其他作物。为进一步开展花生遗传研究，有必要寻找更多的DNA标记。利用SSR富集技术开发了栽培花生的微卫星标记。结果表明，GA/CT重复序列是花生中分布最广的微卫星。引物对设计了五十六个不同的微卫星，其中19个表现出多态性的基因型之间的研究。每个基因座的平均等位基因数为4.25个，一个基因座上最多可发现14个等位基因。这表明微卫星标记在栽培花生中产生的DNA多态性水平高于其他标记。在花生栽培种中分离和鉴定更多的DNA标记，为花生遗传作图、标记辅助选择和基因发现等基因组学研究提供依据。微卫星标记的发展为此类研究提供了希望。
Genomic research of cultivated peanut has lagged behind other crop species because of the paucity of polymorphic DNA markers found in this crop. It is necessary to identify additional DNA markers for further genetic research in peanut. Microsatellite markers in cultivated peanut were developed using the SSR enrichment procedure. The results showed that the GA/CT repeat was the most frequently dispersed microsatellite in peanut. The primer pairs were designed for fifty-six different microsatellites, 19 of which showed a polymorphism among the genotypes studied. The average number of alleles per locus was 4.25, and up to 14 alleles were found at one locus. This suggests that microsatellite DNA markers produce a higher level of DNA polymorphism than other DNA markers in cultivated peanut. It is desirable to isolate and characterize more DNA markers in cultivated peanut for more productive genomic studies, such as genetic mapping, marker-assisted selection, and gene discovery. The development of microsatellite markers holds a promise for such studies.