Assessment of poliovirus eradication in Japan: Genomic analysis of polioviruses isolated from river water and sewage in Toyama Prefecture
Assessment of poliovirus eradication in Japan: Genomic analysis of polioviruses isolated from river water and sewage in Toyama Prefecture
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DOI：
10.1128/aem.66.11.5087-5091.2000
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发表时间：
2000-11-01
影响因子：
4.4
通讯作者：
Kitamura, T
中科院分区：
文献类型：
--
作者：
Matsuura, K;Ishikura, M;Kitamura, T
采用聚合酶链反应限制性片段长度多态性（pcr -限制性片段长度多态性，RFLP）方法对1993 ~ 1995年从日本富山县河水和污水中分离的78株脊髓灰质炎病毒进行了鉴定，并对病毒基因组VP3和VP1区域进行了部分测序。其中，36株被鉴定为Sabin疫苗株，42株被鉴定为与Sabin菌株核苷酸差异小于1.4%的疫苗变异株，其中7株经PCR-RFLP分析与Sabin菌株的模式不同。这些结果表明野生型脊髓灰质炎病毒并未在富山县流行。
Seventy-eight poliovirus strains isolated from river water and sewage in Toyama Prefecture, Japan, during 1993 to 1995 were characterized by the PCR-restriction fragment length polymorphism (RFLP) method and by partially sequencing the VP3 and VP1 regions of the viral genome. Of these isolates, 36 were identified as Sabin vaccine strains, and 42 were identified as vaccine variant strains that had less than 1.4% nucleotide divergence from the Sabin strains, including 7 isolates with patterns different from those of Sabin strains as determined by PCR-RFLP analysis. These findings suggest that wild-type poliovirus was not circulating in Toyama Prefecture.