Reflection contributions to the dispersion artefact in FTIR spectra of single biological cells
Reflection contributions to the dispersion artefact in FTIR spectra of single biological cells
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DOI：
10.1039/b821349f
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发表时间：
2009-01-01
期刊：
影响因子：
4.2
通讯作者：
Gardner, Peter
中科院分区：
文献类型：
--
作者：
Bassan, Paul;Byrne, Hugh J.;Gardner, Peter
透射反射几何结构中单个细胞的傅里叶变换红外光谱随着细胞上的位置而显着变化，在光密核区域显示出扭曲的导数状线形。在溴化钾盘中掺杂的蛋白质白蛋白的模型系统中可以观察到类似的行为。结果表明，任何点的光谱都是样品反射和透射的加权和，并且光学密集区域中反射光谱的主导地位可以解释之前归因于色散伪影的一些光谱失真。反射贡献始终存在于透射反射光谱中，而不是人工制品，并且进一步证明反射特性可用于细胞映射。
Fourier transform infrared spectra of a single cell in transflection geometry are seen to vary significantly with position on the cell, showing a distorted derivative-like lineshape in the region of the optically dense nucleus. A similar behaviour is observable in a model system of the protein albumin doped in a potassium bromide disk. It is demonstrated that the spectrum at any point is a weighted sum of the sample reflection and transmission and that the dominance of the reflection spectrum in optically dense regions can account for some of the spectral distortions previously attributed to dispersion artefacts. Rather than being an artefact, the reflection contribution is ever present in transflection spectra and it is further demonstrated that the reflection characteristics can be used for cellular mapping.