Segmental bronchial provocation induces nasal inflammation in allergic rhinitis patients
Segmental bronchial provocation induces nasal inflammation in allergic rhinitis patients
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DOI：
10.1164/ajrccm.161.6.9906121
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发表时间：
2000-06-01
影响因子：
24.7
通讯作者：
Fokkens, WJ
中科院分区：
文献类型：
--
作者：
Braunstahl, GJ;Kleinjan, A;Fokkens, WJ
过敏性鼻炎和哮喘通常共存，并共享遗传背景。鼻子和肺之间的病理生理联系仍然不完全清楚。本研究旨在比较非哮喘性变应性鼻炎患者节段性支气管激发（SBP）后上、下呼吸道的变应性炎症和临床表现。八名非哮喘，草花粉敏感的过敏性鼻炎患者和八名健康对照。在SBP前（T-0）和SBP后24 h（T-24）采集支气管活检和血液样本。在T-0、SEP后1 h（T-1）和T-24获得鼻活检。对嗜酸性粒细胞（BMK 13）、白细胞介素（IL）-5和eotaxin进行免疫组织化学染色。T-24时，特应性受试者激发和未激发支气管粘膜（p < 0.05）和血液（p = 0.03）中嗜酸性粒细胞数量增加。我们检测到鼻固有层中BMK 13阳性和嗜酸性粒细胞趋化因子阳性细胞的增加，并且仅在T-24时特应性受试者鼻上皮中IL-5的表达增强（p < 0.05）。SEP诱发鼻和支气管症状，以及肺和鼻功能在过敏组的减少。在健康对照组中未观察到显著变化。研究表明，非哮喘性变应性鼻炎患者的SEP可导致外周血嗜酸性粒细胞增多，SEP可诱发鼻内变应性炎症。
Allergic rhinitis and asthma often coexist and share a genetic background. Pathophysiologic connections between the nose and lungs are still not entirely understood. This study was undertaken to compare allergic inflammation and clinical findings in the upper and lower airways after segmental bronchial provocation (SBP) in nonasthmatic allergic rhinitis patients. Eight nonasthmatic, grass pollen-sensitive patients with allergic rhinitis and eight healthy controls were included. Bronchial biopsies and blood samples were taken before (T-0) and 24 h (T-24) after SBP. Nasal biopsies were obtained at T-0, 1 h after SEP (T-1), and T-24. Immunohistochemical staining was performed for eosinophils (BMK13), interleukin (IL)-5, and eotaxin. The number of eosinophils increased in the challenged and unchallenged bronchial mucosa (p < 0.05) and in the blood (p = 0.03) of atopic subjects at T-24. We detected an increase of BMK13-positive and eotaxin-positive cells in the nasal lamina propria and enhanced expression of IL-5 in the nasal epithelium of atopic subjects only at T-24 (p < 0.05). SEP induced nasal and bronchial symptoms as well as reductions in pulmonary and nasal function in the allergic group. No significant changes could be observed in healthy controls. The study shows that SEP in nonasthmatic allergic rhinitis patients results in peripheral blood eosinophilia, and that SEP can induce allergic inflammation in the nose.