Internal Light Source-Driven Photoelectrochemical 3D-rGO/Cellulose Device Based on Cascade DNA Amplification Strategy Integrating Target Analog Chain and DNA Mimic Enzyme
Internal Light Source-Driven Photoelectrochemical 3D-rGO/Cellulose Device Based on Cascade DNA Amplification Strategy Integrating Target Analog Chain and DNA Mimic Enzyme
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基于集成目标模拟链和 DNA 模拟酶的级联 DNA 扩增策略的内部光源驱动光电化学 3D-rGO/纤维素器件
DOI：
10.1021/acsami.7b12338
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发表时间：
2017
影响因子：
9.5
通讯作者：
Yu Jinghua
中科院分区：
文献类型：
--
作者：
Lan Feifei;Liang Linlin;Zhang Yan;Li Li;Ren Na;Yan Mei;Ge Shenguang;Yu Jinghua
在本工作中，我们展示了一种化学发光驱动的中空通道可折叠贺卡状光电化学实验室(GPECD)，其中巧妙地引入了靶触发的级联DNA扩增策略。GPECD具有试剂储存和信号采集的功能，构型的改变可以控制流体路径、反应时间和电性连接的变化。此外，还在纸纤维上原位生长了三维还原氧化石墨烯-金花，以获得良好的导电性和生物相容性。级联DNA扩增策略是指目标类似物链的循环形成及其对杂交链反应(HCR)的触发作用，导致在氯化血红素存在下形成大量的氯化血红素/G-四链DNA模拟酶。在氯化高铁血红素/G-四链体的催化作用下，鲁米诺-H_2O_2体系产生较强的化学发光，并作为内光源激发光活性物质，实现仪器的简化。在这个分析过程中，凝血酶作为概念验证，通过对适体蛋白的特异性识别和目标类似物链的循环，将靶浓度转化为DNA信号输出。在靶标存在的情况下，靶标模拟链大量产生，进而引发了大量的HCR，并将氯化血红素/G-四链体引入系统。用化学发光激发氮掺杂碳点敏化氧化锌后，得到光电流信号。GPECD对凝血酶具有良好的特异性和敏感性，检测下限为16.7fM。这种精心设计的GPECD为生物分析和临床生物医学中检测其他微量蛋白质铺平了一条光明的道路。
In this work, a chemiluminescence-driven collapsible greeting card-like photoelectrochemical lab-on-paper device (GPECD) with hollow channel was demonstrated, in which target-triggering cascade DNA amplification strategy was ingeniously introduced. The GPECD had the functions of reagents storage and signal collection, and the change of configuration could control fluidic path, reaction time and alterations in electrical connectivity. In addition, three-dimentional reduced graphene oxide affixed Au flower was in situ grown on paper cellulose fiber for achieving excellent conductivity and biocompatibility. The cascade DNA amplification strategy referred to the cyclic formation of target analog chain and its trigger action to hybridization chain reaction (HCR), leading to the formation of numerous hemin/G-quadruplex DNA mimic enzyme with the presence of hemin. Subjected to the catalysis of hemin/G-quadruplex, the strong chemiluminiscence of luminol–H2O2system was obtained, which then was used as internal light source to excite photoactive materials realizing the simplification of instrument. In this analyzing process, thrombin served as proof-of-concept, and the concentration of target was converted into the DNA signal output by the specific recognition of aptamer-protein and target analog chain recycling. The target analog chain was produced in quantity with the presence of target, which further triggered abundant HCR and introduced hemin/G-quadruplex into the system. The photocurrent signal was obtained after the nitrogen-doped carbon dots sensitized ZnO was stimulated by chemiluminescence. The proposed GPECD exhibited excellent specificity and sensitivity toward thrombin with a detection limit of 16.7 fM. This judiciously engineered GPECD paved a luciferous way for detecting other protein with trace amounts in bioanalysis and clinical biomedicine.