FLUORESCENT TETRAMETHYL RHODAMINE DERIVATIVES OF ALPHA-BUNGAROTOXIN - PREPARATION, SEPARATION, AND CHARACTERIZATION
FLUORESCENT TETRAMETHYL RHODAMINE DERIVATIVES OF ALPHA-BUNGAROTOXIN - PREPARATION, SEPARATION, AND CHARACTERIZATION
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DOI：
10.1016/0003-2697(77)90682-0
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发表时间：
1977-01-01
影响因子：
2.9
通讯作者：
AXELROD, D
中科院分区：
文献类型：
--
作者：
RAVDIN, P;AXELROD, D
.alpha。-Bungarotoxin [Bungorus multicinctus]用异硫氰酸四甲基罗丹明荧光标记，然后在Sephadex G-25和CM-Sephadex C-50柱上分离。CM-Sephadex G-50色谱柱的洗脱谱显示4个不同的荧光峰和一个未标记的毒素峰。4个荧光峰均可对小鼠隔膜电机端板进行荧光染色。最慢的洗脱峰，峰IV，具有最高的量子效率。峰IV，被鉴定为单标签四甲基罗丹明。α。-班加罗毒素，与电火花片段上的乙酰胆碱受体不可逆结合，并比峰I-III更强烈地标记片段，峰I-III被鉴定为多重标记的四甲基罗丹明混合物。
.alpha.-Bungarotoxin [Bungorus multicinctus] is fluorescently labeled with tetramethyl rhodamine isothiocyanate and then fractionated on Sephadex G-25 and CM-Sephadex C-50 columns. The elution profile of the CM-Sephadex G-50 columns exhibits 4 distinct fluorescent peaks and a peak of unlabeled toxin. All 4 fluorescent peaks can fluorescently stain mouse diaphragm motor end plates. The most slowly eluting peak, Peak IV, has the highest quantum efficiency. Peak IV, which is identified as monolabeled tetramethyl rhodamine .alpha.-bungarotoxin, binds irreversibly to acetylcholine receptors on electroplax fragments and labels the fragments more intensely than Peaks I-III, which are identified as mixtures of multiply labeled tetramethyl rhodamine .alpha.-bungarotoxin.