Differential proteomic analysis of neonatal cardiomyocytes in response to β-adrenergic receptor stimulation
Differential proteomic analysis of neonatal cardiomyocytes in response to β-adrenergic receptor stimulation
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DOI：
10.1016/s1005-9040(06)60107-4
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发表时间：
2006-05-01
影响因子：
3.1
通讯作者：
Zhang You-yi
中科院分区：
文献类型：
--
作者：
Li Zi-jian;Liu Ning;Zhang You-yi
在生理和病理条件下，β-肾上腺素能受体(β-AR)在心脏功能调节中发挥重要作用。为了进一步探讨β-Ars发挥作用的机制，采用蛋白质组学方法研究了异丙肾上腺素(ISO)对培养的乳鼠心肌细胞的整体蛋白质模式的影响。为了提高双向电泳法的重复性和分辨率，采用了一种改进的方法，即“一胶成镜”。获得了重复性较好的2-DE图谱，共检测到1281 70个点，约1191个+/-54个点匹配，平均匹配率为92.9%。利用MALDI-MS获得的多肽质量指纹图谱(PMF)数据，鉴定了9个有显著变化的蛋白质。
beta-Adrenoceptors(beta-ARs) play a critical role in regulating cardiac functions under both physiological and pathological conditions. To further explore the mechanisms through which beta-ARs perform its actions, proteomic approaches were adopted to study the global protein patterns in cultured neonatal rat cardiomyocytes exposed to isoproterenol (ISO). A modified method, "Mirror Images in One Gel", was used to improve the reproducibility and resolution power of two-dimensional electrophoresis. A 2-DE map with a good reproducibility was obtained in which 1281 70 spots were detected and about 1191 +/- 54 spots were matched, with an average matching rate of 92.9%. Nine proteins with significant changes were identified by using peptide mass fingerprinting(PMF) data obtained via MALDI-MS.