A multicatalytic protease complex from pituitary that forms enkephalin and enkephalin containing peptides.
A multicatalytic protease complex from pituitary that forms enkephalin and enkephalin containing peptides.
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DOI：
10.1016/0006-291x(81)91823-4
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发表时间：
1981-08
影响因子：
3.1
通讯作者：
Narian Orlowski;Sherwin Wilk
中科院分区：
文献类型：
--
作者：
Narian Orlowski;Sherwin Wilk
垂体含有高分子量（M.W.∼700,000）中性内肽酶复合物，其在溶液中表现为单一蛋白质，但在解离条件下的聚丙烯酰胺凝胶电泳显示存在分子量为24,000至28,000的五种成分。该复合物表现出胰蛋白酶样、胰凝乳蛋白酶样和肽基谷氨酰-肽键水解活性。实验表明，这些活动中的每一项都与复合体的单独组成部分相关。胰凝乳蛋白酶样和胰蛋白酶样活性从单一合成前体有效生成亮氨酸脑啡肽、亮氨酸脑啡肽-Arg6 和亮氨酸脑啡肽-Arg6-Phe7。胰凝乳蛋白酶样活性的选择性抑制增强胰蛋白酶样活性并改变所形成的阿片样肽的相对比例。
The pituitary contains a high molecular weight (M.W.∼700,000) neutral endopeptidase complex that behaves in solution as a single protein, but on polyacrylamide gel electrophoresis under dissociating conditions shows the presence of five components with molecular weights of 24,000 to 28,000. The complex exhibits trypsinlike, chymotrypsinlike and peptidylglutamyl-peptide bond hydrolysing activities. Experiments indicate that each of these activities is associated with a separate component of the complex. The chymotrypsinlike and trypsinlike activities efficiently generate Leu-enkephalin, Leu-enkephalin-Arg6and Leu-enkephalin-Arg6-Phe7from a single synthetic precursor. Selective inhibition of the chymotrypsinlike activity enhances the trypsinlike activity and alters the relative proportion of opioid peptides formed.