Isotopic analysis of epidermal mucus in freshwater fishes can reveal short-time diet variations

Isotopic analysis of epidermal mucus in freshwater fishes can reveal short-time diet variations
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淡水鱼表皮粘液的同位素分析可以揭示短期饮食变化

DOI:
10.1007/s11284-017-1478-8
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发表时间:
2017
影响因子:
2
通讯作者:
Maruyama Atsushi
Maruyama Atsushi
中科院分区:
环境科学与生态学4区
文献类型:
--
作者:
Shigeta Kanji;Tsuma Shiori;Yonekura Ryuji;Kakamu Hiroto;Maruyama Atsushi

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虽然稳定同位素分析是一个强大的工具,用于确定动物的饮食,迁移模式,以及在水生系统中的食物网的结构,在传统上使用的鱼类肌肉组织中的同位素比率的缓慢反应往往阻碍这种方法。通过分析同位素变化更快的组织,这种技术可以在比传统同位素分析更精细的时间尺度上使用。通过饲料转换实验,比较了3种淡水鲤科鱼类表皮粘液、鳍和肌肉组织中稳定碳、氮同位素比值(δ 13 C和δ 15 N)的变化。同位素变化的半衰期在粘液中为62至144天,在鳍中为57至202天,在肌肉组织中为196至680天。粘液中的同位素比变化更迅速,比在肌肉组织中的三个物种的检查,类似于以前研究的三个物种(鲶鱼,钢头,淡水虾虎鱼),而鳍显示相对可变的趋势同位素变化。与其它两种组织相比,鳍组织的δ 13 C值在营养辨别因子(TDF)上的种间变异较大。一般快速变化和相对恒定的TDF,在同位素分析中使用表皮粘液,而不是广泛使用的鳍和肌肉组织,有利于研究鱼类的食物习性和食物网结构的短期变化。
Although stable isotope analysis is a powerful tool for determining animal diets, migration patterns, and the structure of food webs in aquatic systems, the slow response of isotopic ratios in the traditionally used muscle tissue of fishes often hampers this approach. By analyzing tissues with more rapid isotopic change, this technique can be used over a finer time scale than traditional isotopic analyses. We conducted a diet-switch experiment to compare changes in the stable carbon and nitrogen isotope ratio values (δ13C andδ15N, respectively) in epidermal mucus, fin and muscle tissues of three species of freshwater cyprinid fishes. The half-life of the isotopic change ranged from 62 to 144 days in mucus, 57 to 202 days in fin, and 196 to 680 days in the muscle tissue. The isotope ratios in mucus changed more rapidly than those in muscle tissues in the three species examined, similarly to the previously studied three species (catfish, steelhead, freshwater goby), whereas fin showed relatively variable trends in isotopic change. Interspecific variation in the trophic discrimination factor (TDF) was particularly large in theδ13C values of fin tissue compared with those in the other two tissues. With generally rapid change and relatively invariable TDF, the use of epidermal mucus in isotope analysis instead of the widely used fin and muscle tissues is advantageous for studying short-term changes in food habits of fishes and food web structures.
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