Nanobody-based VSR7 tracing shows clathrin-dependent TGN to Golgi recycling.
Nanobody-based VSR7 tracing shows clathrin-dependent TGN to Golgi recycling.
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DOI：
10.1038/s41467-023-42331-1
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发表时间：
2023-10-30
影响因子：
16.6
通讯作者：
Pimpl, Peter
中科院分区：
文献类型：
--
作者：
Shao, Xiaoyu;Xu, Hao;Pimpl, Peter
受体介导的可溶性蛋白转运是自然界赋予真核细胞获取大量大分子的关键，无论是通过直接积累还是作为生化途径的产物。这些机制的运输效率一方面源于受体捕获、运输和释放配体的能力，另一方面源于允许进行多轮配体运输的循环能力。然而，植物液泡分选受体（VACUOLAR SORTING RECEPTOR， VSR）蛋白家族是多种多样的，其配体特异性、双向转运途径和转运机制仍存在很大争议。在这里，我们采用基于纳米体表位相互作用的分子工具来评估vsr7在体内的功能。我们证明了VSR7对序列特异性液泡分选信号的特异性，并追踪了它的顺行运输和逆行回收路线。VSR7在稳态条件下定位于顺式高尔基体，并在TGN/EE进行网格蛋白依赖的再循环之前，将配体向下游运输并释放到反式高尔基网络/早期内体（TGN/EE）中。基于纳米体-表位相互作用的拟南芥液泡分类受体7 （VSR7）分析表明，由于酸驱动的配体释放和网格蛋白依赖的受体循环，基于固定的配体运输到TGN/EE和末端。
Receptor-mediated transport of soluble proteins is nature’s key to empowering eukaryotic cells to access a plethora of macromolecules, either by direct accumulation or as products from resulting biochemical pathways. The transport efficiency of these mechanisms results from the receptor’s capability to capture, transport, and release ligands on the one hand and the cycling ability that allows for performing multiple rounds of ligand transport on the other. However, the plant VACUOLAR SORTING RECEPTOR (VSR) protein family is diverse, and their ligand-specificity and bidirectional trafficking routes and transport mechanisms remain highly controversial. Here we employ nanobody-epitope interaction-based molecular tools to assess the function of the VSR 7 in vivo. We demonstrate the specificity of the VSR7 for sequence-specific vacuolar sorting signals, and we trace its anterograde transport and retrograde recycling route. VSR7 localizes at the cis-Golgi apparatus at steady state conditions and transports ligands downstream to release them in the trans-Golgi network/early endosome (TGN/EE) before undergoing clathrin-dependent recycling from the TGN/EE back to the cis-Golgi. Nanobody-epitope interaction-based analysis of the Arabidopsis VACUOLAR SORTING RECEPTOR7 (VSR7) suggests immobilization-based ligand transport to the TGN/EE and ends due to acidity-driven ligand release and clathrin-dependent receptor recycling.
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影响因子：
5.6
作者：
Martinière A;Desbrosses G;Sentenac H;Paris N
通讯作者：
Paris N
影响因子：
11.6
作者：
HOLWERDA, BC;PADGETT, HS;ROGERS, JC
通讯作者：
ROGERS, JC
DOI：
10.1083/jcb.200504112
发表时间：
2005-08-29
期刊：
The Journal of cell biology
影响因子：
--
作者：
Park M;Lee D;Lee GJ;Hwang I
通讯作者：
Hwang I
影响因子：
11.6
作者：
Lam, Sheung Kwan;Siu, Ching Lung;Jiang, Liwen
通讯作者：
Jiang, Liwen
影响因子：
11.6
作者：
Kang, Hyangju;Kim, Soo Youn;Hwang, Inhwan
通讯作者：
Hwang, Inhwan