Comparative analysis of hyaluronan's affinity for antivascular endothelial growth factor agents.
Comparative analysis of hyaluronan's affinity for antivascular endothelial growth factor agents.
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透明质酸与抗血管内皮生长因子药物的亲和力的比较分析。
DOI：
10.1159/000342975
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Zako M.
中科院分区：
文献类型：
--
作者：
Sugita I;Yoneda M;Iwaki M;Zako M.
背景贝伐单抗是一种抗血管内皮生长因子（VEGF）的药物，当注射到玻璃体切除和非玻璃体切除眼的玻璃体腔内时，其对视网膜病变伴新生血管形成的疗效差异提示玻璃体的主要成分透明质酸的参与。本研究的目的是比较透明质酸的亲和力抗VEGF agents in vitro.MethodsWe研究了透明质酸的亲和力3抗VEGF剂（贝伐单抗，哌加他尼和雷珠单抗）。氚[3H]标记的透明质酸分别与每种抗VEGF剂孵育。结合和未结合的透明质酸测量使用固相和液相methods的比率calculated.ResultsHyaluronan表现出显着更大的亲和力贝伐单抗比哌加他尼或ranibizumab.ConclusionsThe不存在或存在的透明质酸可能与贝伐单抗注射到玻璃体腔的临床疗效，由于贝伐单抗的亲和力的透明质酸。
BackgroundDifferences in the efficacy of bevacizumab, an antivascular endothelial growth factor (VEGF) agent, against retinopathy with neovascularization when injected into the vitreous cavity of vitrectomized and nonvitrectomized eyes suggests the involvement of hyaluronan, a major component of the vitreous body. This study aimed to compare the affinities of hyaluronan for anti-VEGF agents in vitro.MethodsWe examined the affinities of hyaluronan for 3 anti-VEGF agents (bevacizumab, pegaptanib and ranibizumab). Tritium [3 H]-labeled hyaluronan was incubated separately with each anti-VEGF agent. The ratio of bound and unbound hyaluronan measured using solid and liquid phase methods was calculated.ResultsHyaluronan demonstrated a significantly greater affinity for bevacizumab than for pegaptanib or ranibizumab.ConclusionsThe absence or presence of hyaluronan may be associated with the clinical efficacy of bevacizumab injected into the vitreous cavity due to the affinity of hyaluronan for bevacizumab.