Identification of Bombyx mori Akt and its phosphorylation by bombyxin stimulation
Identification of Bombyx mori Akt and its phosphorylation by bombyxin stimulation
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DOI：
10.1016/j.cbpb.2008.08.002
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发表时间：
2008-11-01
影响因子：
2.2
通讯作者：
Nagasawa, Hiromichi
中科院分区：
文献类型：
--
作者：
Nagata, Shinji;Hakuno, Fumihiko;Nagasawa, Hiromichi
Akt是一种参与胰岛素信号传导的丝氨酸/苏氨酸蛋白激酶，从家蚕（Bombyx mori）中鉴定出来。Bombyx Akt （BomAkt）由493个氨基酸残基组成，包括在其他Akt中保守的区域：Pleckstrin同源结构域和激酶结构域，以及激酶激活必需的双磷酸化位点。市售的抗哺乳动物Akt和phosphoAkt抗体能够在表达BomAkt的HEK293细胞中识别BomAkt和磷酸化的BomAkt。此外，在表达BomAkt的胰岛素样生长因子(IGF)-I刺激的hek293细胞中可以检测到BomAkt的磷酸化。RT-PCR和免疫印迹分析显示，BomAkt在家蚕幼虫中普遍表达。BomAkt的磷酸化在暴露于bombyxin（一种内源性胰岛素样肽）后的离体脂肪体和饥饿后重新喂食的幼虫脂肪体中都被观察到。这些结果表明，膳食摄入可能通过家蚕素的作用激活胰岛素信号通路，包括Akt。(C) 2008爱思唯尔公司版权所有。
Akt, a Ser/Thr protein kinase involved in insulin signaling, was identified from the silkworm, Bombyx mori. Bombyx Akt (BomAkt) is composed of 493 amino acid residues including regions conserved in other Akts: the Pleckstrin homology and kinase domains, and a dual phosphorylation site essential for kinase activation. Commercially available antibodies against mammalian Akt and phosphoAkt were able to recognize BomAkt and phosphorylated BomAkt in HEK293 cells expressing BomAkt. Additionally, phosphorylation of BomAkt was detectable in insulin-like growth factor (IGF)-I stimulated-HEK293 cells expressing BomAkt. RT-PCR and immunoblotting analyses revealed that BomAkt is expressed ubiquitously in Bombyx larvae. Phosphorylation of BomAkt was observed both in the isolated fat body after exposure to bombyxin, an endogenous insulin-like peptide, and in the larval fat body by refeeding a diet after starvation. These results suggest that dietary intake may activate the insulin signaling pathway, including Akt, through bombyxin action in B. mori. (C) 2008 Elsevier Inc. All rights reserved.