Inhibitory effect of caffeic acid on cancer cell proliferation by oxidative mechanism in human HT-1080 fibrosarcoma cell line
Inhibitory effect of caffeic acid on cancer cell proliferation by oxidative mechanism in human HT-1080 fibrosarcoma cell line
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DOI：
10.1007/s11010-010-0655-7
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发表时间：
2011-03-01
影响因子：
4.3
通讯作者：
Reddy, Bandugula Venkata
中科院分区：
文献类型：
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作者：
Prasad, N. Rajendra;Karthikeyan, A.;Reddy, Bandugula Venkata
咖啡酸（3,4-二羟基肉桂酸）(CA) 天然存在于水果、蔬菜、橄榄油和咖啡中。本研究旨在评估咖啡酸对 HT-1080 人纤维肉瘤细胞系的抗癌作用。通过MTT法测定咖啡酸的抗增殖作用，通过脂质过氧化、酶和非酶抗氧化状态的变化测定氧化应激。为了了解CA的抗增殖作用模式，作者通过DCFH-DA方法观察细胞内ROS水平，通过Rh-123染色观察线粒体膜电位变化，通过彗星实验观察氧化DNA损伤，并通过AO/EtBr染色方法观察细胞凋亡形态变化。结果表明，咖啡酸可增强 HT-1080 细胞系中的脂质过氧化标志物，如 TBARS、CD 和 LHP。咖啡酸可提高 ROS 水平，DCF 荧光增加证明了这一点。此外，咖啡酸处理改变了 HT-1080 细胞中的线粒体膜电位。同样，作者观察到咖啡酸处理组中氧化 DNA 损伤（% 尾部 DNA、% 尾部长度、尾矩和橄榄尾矩）增加，以及细胞凋亡形态变化。这些数据表明咖啡酸在 HT-1080 细胞系中表现出有效的抗癌作用，并且它可以用作抗癌剂。
Caffeic acid (3,4-dihydroxy cinnamic acid) (CA) is naturally found in fruits, vegetables, olive oil, and coffee. This study was undertaken to evaluate the anticancer effect of caffeic acid on HT-1080 human fibrosarcoma cell line. The antiproliferative effect of caffeic acid was determined by MTT assay, and the oxidative stress was determined by lipid peroxidation, changes in the enzymatic, and non-enzymatic antioxidant status. To understand the mode of antiproliferative effect of CA, the authors observed intracellular ROS levels by DCFH-DA method, mitochondrial membrane potential alterations by Rh-123 staining, oxidative DNA damage by comet assay, and apoptotic morphological changes by AO/EtBr-staining method. The results show that caffeic acid enhances lipid peroxidative markers such as TBARS, CD, and LHP in HT-1080 cell line. Caffeic acid enhances the ROS levels, which is evidenced by the increased DCF fluorescence. Further, caffeic acid treatment altered the mitochondrial membrane potential in HT-1080 cells. Similarly, the authors observed increased oxidative DNA damage (% Tail DNA, % Tail length, Tail moment, and olive tail moment), and apoptotic morphological changes in caffeic acid-treated groups. These data suggest that caffeic acid exhibits potent anticancer effect in HT-1080 cell line, and that it may be used as an anticancer agent.