ESCHERICHIA-COLI CELL-DIVISION PROTEIN FTSZ IS A GUANINE-NUCLEOTIDE BINDING-PROTEIN
ESCHERICHIA-COLI CELL-DIVISION PROTEIN FTSZ IS A GUANINE-NUCLEOTIDE BINDING-PROTEIN
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DOI：
10.1073/pnas.90.3.1053
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发表时间：
1993-02-01
影响因子：
11.1
通讯作者：
LUTKENHAUS, J
中科院分区：
文献类型：
--
作者：
MUKHERJEE, A;DAI, K;LUTKENHAUS, J
FtsZ是大肠杆菌中一种重要的细胞分裂蛋白，在细胞周期调控下，在分裂部位形成环状结构。FtsZ环的动态性质表明它可能与真核细胞中的微丝形成蛋白如微管蛋白相似。在本研究中，我们确定FtsZ是一种具有GTP酶活性的GTP/GDP结合蛋白。FtsZ的一小段与微管蛋白中的一段同源，后者被认为参与了微管蛋白和鸟嘌呤核苷酸之间的相互作用。致命性的FtsZ突变ftsZ3(Rsa)导致该同源片段的氨基酸改变，减少了GTP的结合和水解性，表明与GTP的相互作用是FtsZ功能所必需的。
FtsZ is an essential cell division protein in Escherichia coli that forms a ring structure at the division site under cell cycle control. The dynamic nature of the FtsZ ring suggests possible similarities to eukaryotic filament forming proteins such as tubulin. In this study we have determined that FtsZ is a GTP/GDP binding protein with GTPase activity. A short segment of FtsZ is homologous to a segment in tubulin believed to be involved in the interaction between tubulin and guanine nucleotides. A lethal ftsZ mutation, ftsZ3 (Rsa), that leads to an amino acid alteration in this homologous segment decreased GTP binding and hydrolysis, suggesting that interaction with GTP is essential for ftsZ function.