A generalized stoichiometric model of C3, C2, C2+C4, and C4 photosynthetic metabolism.
A generalized stoichiometric model of C3, C2, C2+C4, and C4 photosynthetic metabolism.
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DOI：
10.1093/jxb/erw303
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发表时间：
2017-01
影响因子：
6.9
通讯作者：
Bellasio C
中科院分区：
文献类型：
--
作者：
Bellasio C
完全基于质量平衡约束的模型完善了我们的理解的权衡，能量需求，叶级通量，在不同的生化类型的同化和可塑性机制。抑制作物光呼吸以最大限度地提高同化和产量的目标，正在激发研究人员对C3植物生物工程碳浓缩机制的极大兴趣。然而，在维管束鞘的生化活动的详细量化是缺乏的。这项工作提出了一个一般的化学计量模型C3，C2，C2+C4和C4同化（SMA），其中明确包括能量，代谢物交通，和不同的脱羧酶（NAD依赖的苹果酸酶，NADP依赖的苹果酸酶，或磷酸烯醇丙酮酸羧激酶）。SMA可用于改进实验数据分析或制定假设场景，并在免费提供的Microsoft Excel工作簿中编码。通过一系列模拟分析了理论基础和一般模型行为，包括（i）在操作条件下C3、C2、C2+C4和C4的分析;（ii）操纵C3植物中的光呼吸;（iii）逐步上调C3光合作用中的C2穿梭;（iv）逐步上调C2光合作用中的C4循环;（iv）逐步上调C2光合作用中的C4循环。以及（v）操纵被假设为响应瞬时环境输入的过程。结果量化了功能权衡，如满足ATP/NADPH需求所需的电子传递，以及不同亚型固有的代谢物交通。SMA改进了我们对光合作用化学计量的理解，这对基础和应用研究至关重要。
A model based solely on mass-balance constraints refines our understanding of the trade-offs, energy requirements, leaf-level fluxes, and plasticity mechanisms in different biochemical types of assimilation. The goal of suppressing photorespiration in crops to maximize assimilation and yield is stimulating considerable interest among researchers looking to bioengineer carbon-concentrating mechanisms into C3 plants. However, detailed quantification of the biochemical activities in the bundle sheath is lacking. This work presents a general stoichiometric model for C3, C2, C2+C4, and C4 assimilation (SMA) in which energetics, metabolite traffic, and the different decarboxylating enzymes (NAD-dependent malic enzyme, NADP-dependent malic enzyme, or phosphoenolpyruvate carboxykinase) are explicitly included. The SMA can be used to refine experimental data analysis or formulate hypothetical scenarios, and is coded in a freely available Microsoft Excel workbook. The theoretical underpinnings and general model behaviour are analysed with a range of simulations, including (i) an analysis of C3, C2, C2+C4, and C4 in operational conditions; (ii) manipulating photorespiration in a C3 plant; (iii) progressively upregulating a C2 shuttle in C3 photosynthesis; (iv) progressively upregulating a C4 cycle in C2 photosynthesis; and (v) manipulating processes that are hypothesized to respond to transient environmental inputs. Results quantify the functional trade-offs, such as the electron transport needed to meet ATP/NADPH demand, as well as metabolite traffic, inherent to different subtypes. The SMA refines our understanding of the stoichiometry of photosynthesis, which is of paramount importance for basic and applied research.
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影响因子：
6.9
作者：
Bellasio C;Burgess SJ;Griffiths H;Hibberd JM
通讯作者：
Hibberd JM
影响因子：
7.4
作者：
Dal'Molin, Cristiana Gomes de Oliveira;Quek, Lake-Ee;Nielsen, Lars Keld
通讯作者：
Nielsen, Lars Keld
影响因子：
6.9
作者：
Keerberg O;Pärnik T;Ivanova H;Bassüner B;Bauwe H
通讯作者：
Bauwe H
影响因子：
7.3
作者：
Buckley, Thomas N.;Adams, Mark A.
通讯作者：
Adams, Mark A.
影响因子：
6.9
作者：
Furbank, Robert T.
通讯作者：
Furbank, Robert T.