Effects of the flavonoids quercetin and apigenin on hemostasis in healthy volunteers: results from an in vitro and a dietary supplement study
Effects of the flavonoids quercetin and apigenin on hemostasis in healthy volunteers: results from an in vitro and a dietary supplement study
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DOI：
10.1093/ajcn/67.2.255
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发表时间：
1998-02-01
影响因子：
7.1
通讯作者：
Katan, MB
中科院分区：
文献类型：
--
作者：
Janssen, PLTMK;Mensink, RP;Katan, MB
在一些流行病学研究中，膳食黄酮醇和黄酮的摄入与心血管疾病的风险呈负相关。这可能是由于黄酮类化合物对止血的作用，因为据报道黄酮类化合物在体外抑制血小板聚集。我们确实发现2500 μ mol/L的黄酮醇槲皮素和黄酮芹菜素显著抑制胶原和adp诱导的聚集在富血小板血浆和洗血小板中约80-97%。然而，较低的浓度，如可能发生在体内，没有影响。为了在体内测试这一点，我们在随机交叉实验中给18名健康志愿者喂食220克洋葱/天，提供114毫克槲皮素/天，5克干欧芹/天，提供84毫克芹菜素/天，或安慰剂，每次7天，每次治疗期持续2周。洋葱摄入使槲皮素平均血浆浓度升高至1.5 μ mol/L；血浆芹菜素无法测定。没有发现洋葱或欧芹对血小板聚集、血栓素B-2、生成、因子VII或其他止血变量有显著影响。我们得出结论，黄酮类化合物在体外的抗聚集作用是由于体内无法达到的浓度。膳食黄酮醇和黄酮对心血管风险的影响可能不是由止血变量介导的。
Intake of dietary flavonols and flavones was inversely associated with risk for cardiovascular disease in several epidemiologic studies. This may have been due to effects on hemostasis because flavonoids have been reported to inhibit platelet aggregation in vitro. We indeed found that 2500 mu mol/L of the flavonol quercetin and the flavone apigenin significantly inhibited collagen-and ADP-induced aggregation in platelet-rich plasma and washed platelets by approximate to 80-97%. However, lower concentrations, such as might occur in vivo, had no effect. To test this in vivo we fed 18 healthy volunteers 220 g onions/d providing 114 mg quercetin/d, 5 g dried parsley/d providing 84 mg apigenin/d, or a placebo for 7 d each in a randomized crossover experiment with each treatment period lasting 2 wk. Onion consumption raised mean plasma quercetin concentrations to 1.5 mu mol/L; plasma apigenin could not be measured. No significant effects of onions or parsley were found on platelet aggregation, thromboxane B-2, production, factor VII, or other hemostatic variables. We conclude that the antiaggregatory effects of flavonoids seen in vitro are due to concentrations that cannot be attained in vivo. Effects of dietary flavonols and flavones on cardiovascular risk are possibly not mediated by hemostatic variables.