Gut Microbiota and Metabolome Response of Decaisnea insignis Seed Oil on Metabolism Disorder Induced by Excess Alcohol Consumption
Gut Microbiota and Metabolome Response of Decaisnea insignis Seed Oil on Metabolism Disorder Induced by Excess Alcohol Consumption
复制标题
十藻籽油对过量饮酒引起的代谢紊乱的肠道微生物群和代谢组反应
DOI：
10.1021/acs.jafc.9b04792
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发表时间：
2019
影响因子：
6.1
通讯作者：
Zhao Yan
中科院分区：
文献类型：
--
作者：
Liu Xiaoxia;Zhao Ke;Yang Xingbin;Zhao Yan
本实验研究了富含棕榈油酸（55.25%）、棕榈酸（12.25%）和油酸（28.74%）的柏树籽油（DISO）对小鼠酒精性代谢紊乱的调节作用。50只小鼠口服38%酒精（0.4 mL/天），不含或含DISO(3、6和12 g/kg)，连续12周。DISO抑制了酒精引起的体重下降和肝功能异常（p< 0.01），改变了盲肠微生物群的分布：提高了乳酸菌、瘤胃菌群（Ruminoccoceae_UCG_004）的丰度（p< 0.05），降低了副杆菌的丰度（p< 0.05）。该处理还调节盲肠内容物中氨基酸和脂质代谢的代谢组反应：上调5-羟基吲哚-3-乙酸（p< 0.05）、6-羟基烟酸、5-甲氧基色胺、烟酰胺和烟酸（p< 0.1），下调雄酮、色氨酸和吲哚-3-乙酰胺（p< 0.05）。DISO通过增加乳酸菌的相对丰度来防止酒精性肝损伤和肠道微生物群失调，这与改善肠道通透性和色氨酸代谢呈正相关。
This study investigated the modulatory effects ofDecaisnea insignisseed oil (DISO), which was rich in palmitoleic acid (55.25%), palmitic acid (12.25%), and oleic acid (28.74%), on alcohol-induced metabolism disorder in mice. Fifty mice were orally administered with 38% alcohol (0.4 mL/day) and without or with DISO (3, 6, and 12 g/kg) for consecutive 12 weeks. DISO inhibited the alcohol-induced weight loss and liver function abnormality (p< 0.01) and shifted the profiles of cecal microbiome: elevating the abundance ofLactobacillus,Ruminoccoceae_UCG_004 (p< 0.05) and decreasing abundance ofParabacteroides(p< 0.05). This treatment also regulated metabolome response of amino acid and lipid metabolism in cecal content: upregulating 5-hydroxyindole-3-acetic acid (p< 0.05), 6-hydroxynicotinic acid, 5-methoxytryptamine, nicotinamide, and nicotinic acid (p< 0.1) and downregulating androsterone, tryptophan, and indole-3-acetamide (p< 0.05). DISO protected against alcoholic liver injury and gut microbiota dysbiosis by enriching the relative abundance ofLactobacillus, which was positively associated with the improvement of intestinal permeability and tryptophan metabolism.