A digital microfluidic method for dried blood spot analysis
A digital microfluidic method for dried blood spot analysis
复制标题
DOI：
10.1039/c1lc20524b
复制
发表时间：
2011-01-01
期刊：
影响因子：
6.1
通讯作者：
Wheeler, Aaron R.
中科院分区：
文献类型：
--
作者：
Jebrail, Mais J.;Yang, Hao;Wheeler, Aaron R.
以干血斑(DBS)形式存储的血液样本正在成为一种广泛应用的有用的采样和存储工具。不幸的是，DBS样本的迅速流行并没有伴随着提取和分析自动化技术的进步。作为克服这一挑战的第一步，我们开发了一个用于干血斑点氨基酸定量的原型微流控系统，在该系统中，分析物被提取、与内标混合、衍生和重组，以便通过(离线和在线)串联质谱仪进行分析。新方法快速、健壮、精确，最重要的是，与自动化兼容。我们认为，新的方法可能有助于新一代分析技术在广泛的应用中定量DBS样品中的分析物。
Blood samples stored as dried blood spots (DBSs) are emerging as a useful sampling and storage vehicle for a wide range of applications. Unfortunately, the surging popularity of DBS samples has not yet been accompanied by an improvement in automated techniques for extraction and analysis. As a first step towards overcoming this challenge, we have developed a prototype microfluidic system for quantification of amino acids in dried blood spots, in which analytes are extracted, mixed with internal standards, derivatized, and reconstituted for analysis by (off-line and in-line) tandem mass spectrometry. The new method is fast, robust, precise, and most importantly, compatible with automation. We propose that the new method can potentially contribute to a new generation of analytical techniques for quantifying analytes in DBS samples for a wide range of applications.