A single nucleotide polymorphism in the MMP-1 promoter is correlated with histological differentiation of gastric cancer
A single nucleotide polymorphism in the MMP-1 promoter is correlated with histological differentiation of gastric cancer
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DOI：
10.1007/s00432-004-0543-1
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发表时间：
2004-05-01
影响因子：
3.6
通讯作者：
Yasui, W
中科院分区：
文献类型：
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作者：
Matsumura, S;Oue, N;Yasui, W
目的.基质金属蛋白酶-1（MMP-1）通过降解细胞外基质（ECM）和基底膜屏障，在肿瘤的侵袭和转移中起关键作用。MMP-1基因启动子区-1607 bp处的1G/2G单核苷酸多态性（single nucleotide polymorphism，SNP）对MMP-1基因的转录活性有影响。根据这些发现，我们研究了MMP-1启动子中的SNP是否与胃癌的发生、分化和进展相关。方法.胃癌患者215例，对照组166例。采用PCR-RFLP和测序方法分析MMP-1基因启动子区的SNP。比较病例组和对照组的基因型频率，并分析病例组中基因型频率与临床病理参数的关系。结果胃癌患者1G/2G基因型频率与对照组相似（p=0.57）。肿瘤浸润程度、淋巴结转移、临床分期与SNP无明显相关性。另一方面，我们发现胃癌患者的组织分化和性别之间存在显著相关性（p
Purpose. Matrix metalloproteinase-1 (MMP-1) plays a key role in cancer invasion and metastasis by degradation of extracellular matrix (ECM) and basement membrane barriers. The 1G/2G single nucleotide polymorphism (SNP) in the MMP-1 promoter at position -1607 bp has been reported to affect the transcriptional activity. In the light of these findings, we investigated whether this SNP in the MMP-1 promoter is associated with the development, differentiation, and progression of gastric cancer. Methods. The 215 gastric cancer patients and 166 controls were used in this study. The SNP of the MMP-1 promoter was analyzed by PCR-RFLP and sequencing. The genotype frequency was compared between cases and controls, and the association with clinicopathological parameters among cases was studied. Results. The frequency of 1G/2G genotypes in gastric cancer patients was similar to those in controls (p=0.57). The degree of tumor invasion, the presence of lymph node metastasis, and clinical stage showed no significant association with the SNP. On the other hand, we found a significant association with histological differentiation and gender among gastric cancer patients (p